Department of Health and Nutrition, Faculty of Human Life Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, 558-8585, Osaka.
Environ Health Prev Med. 2001 Jan;5(4):138-43. doi: 10.1007/BF02918289.
The effects of a 47-week diet of butter or safflower oil as fat in combination with casein or soy protein as protein were observed for the serum concentrations of lipids and fatty acid compositions in rat serum and heart. Serum total cholesterol (Chol) did not differ among the four experimental diet groups. In the butter groups, significantly higher low-density lipoprotein (LDL)-Chol and lower high-density lipoprotein (HDL)-Chol were observed than in the safflower oil groups (p<0.005, respectively). Higher levels of α-tocopherol were found in the butter groups than in the safflower oil groups (p<0.05) and in the casein groups than in the soy protein groups (p<0.01). In comparison with the safflower oil groups, the butter groups showed higher n-3 polyunsaturated fatty acids (PUFA) contents and lower n-6 PUFA contents in serum and the hearts (p<0.005). The ratios of n-3/n-6 PUFA in the butter groups in serum, 0.26 and 0.18, and in the hearts, 0.37 and 0.36, (butter-casein diet and butter-soy protein diet, respectively) were higher than those of the safflower oil groups of under 0.01 in serum and 0.02 and 0.03 in the hearts (safflower oil-casein diet and safflower oil-soy protein diet, respectively) (p<0.005). In the soy protein groups, higher n-3 PUFA contents in the hearts were found than those of the casein groups (p<0.05). This study suggested that the butter diet induces higher levels of n-3 PUFA and a higher n-3/n-6 PUFA ratio than the safflower oil diet in rat serum and hearts over a long feeding period.
在为期 47 周的实验中，给大鼠喂食黄油或红花籽油作为脂肪，并分别以酪蛋白或大豆蛋白作为蛋白质，观察大鼠血清和心脏中的脂质和脂肪酸组成的血清浓度。四组实验性饮食的血清总胆固醇（Chol）没有差异。在黄油组中，与红花油组相比，低密度脂蛋白胆固醇（LDL-Chol）显著升高，而高密度脂蛋白胆固醇（HDL-Chol）显著降低（分别为 p<0.005）。与红花油组相比，黄油组的α-生育酚水平较高（p<0.05），与大豆蛋白组相比，酪蛋白组的α-生育酚水平较高（p<0.01）。与红花油组相比，黄油组的血清和心脏中的 n-3 多不饱和脂肪酸（PUFA）含量较高，n-6 PUFA 含量较低（p<0.005）。黄油组血清中的 n-3/n-6 PUFA 比值（黄油-酪蛋白饮食和黄油-大豆蛋白饮食，分别为 0.26 和 0.18）和心脏中的 n-3/n-6 PUFA 比值（0.37 和 0.36，黄油-酪蛋白饮食和黄油-大豆蛋白饮食，分别）高于红花油组（血清中的 n-3/n-6 PUFA 比值低于 0.01，心脏中的 n-3/n-6 PUFA 比值为 0.02 和 0.03，红花油-酪蛋白饮食和红花油-大豆蛋白饮食，分别）（p<0.005）。在大豆蛋白组中，心脏中的 n-3 PUFA 含量高于酪蛋白组（p<0.05）。本研究表明，在长期喂养过程中，与红花油饮食相比，黄油饮食可使大鼠血清和心脏中的 n-3 PUFA 水平升高，并使 n-3/n-6 PUFA 比值升高。
