中国燃煤型地方性慢性砷中毒患者尿液中的卟啉
Urinary porphyrins in patients with endemic chronic arsenic poisoning caused by burning coal in China.
机构信息
Department of Emdemiology and Environmental Health, Juntendo University School of Medicine, 1-1-1 Hongo, Bunkyo ku, 13-8421, Tokyo, Japan.
出版信息
Environ Health Prev Med. 2001 Jan;5(4):180-5. doi: 10.1007/BF02918296.
OBJECTIVE
To evaluate the effect of arsenic (As) on the porphyrin biosynthetic pathway, urine samples from patients with endemic chronic arsenic poisoning were examined.
SUBJECTS AND METHODS
The subjects were 16 patients, who had been exposed to As from burning coal for 8 to 25 years, and 16 controls living in the same region in Guizhou Province in southwest China. Concentrations of urinary As, porphyrins and ALA were determined by induced coupled plasma mass spectrometry (ICP-MS), high performance liquid chromatography (HPLC) with a reversed-phase column and fluorescence detector, and colorimetric spectrophotometry, respectively.
RESULTS
Concentrations of As in patients and controls, 184.40±200.04 and 86.82±64.20μ g/g creatinine (mean ±SD) respectively, were significantly different (p<0.05). The concentrations of various kinds of urinary porphyrins, including isomers I and III of coproporphyrin and pentacarboxylporphyrin, were determined. Positive correlations were observed between As and porphyrins (e.g. total porphyrins, hexacarboxylporphyrin and coproporphyrin III) or between As and ALA in male and female patients. However, porphyrin and ALA concentrations were not significantly different between the patients and the controls. Urinary porphyrin concentrations in females were higher than those in males.
CONCLUSION
Exposure to As from burning coal may influence porphyrin biosynthesis.
目的
评估砷（As）对卟啉生物合成途径的影响，检测了来自地方性慢性砷中毒患者的尿液样本。
对象与方法
研究对象为 16 名患者，他们因燃烧煤炭而暴露于 As 8-25 年，以及 16 名来自中国西南部贵州省同一地区的对照者。通过电感耦合等离子体质谱法（ICP-MS）、高效液相色谱法（HPLC）与反相柱和荧光检测器以及分光光度比色法分别测定尿砷、卟啉和 ALA 的浓度。
结果
患者和对照组的 As 浓度分别为 184.40±200.04μg/g 肌酐和 86.82±64.20μg/g 肌酐（均数±标准差），差异有统计学意义（p<0.05）。测定了各种尿卟啉的浓度，包括粪卟啉 I 和 III 异构体和五羧基卟啉。在男性和女性患者中，As 与卟啉（如总卟啉、六羧基卟啉和粪卟啉 III）之间或 As 与 ALA 之间呈正相关。然而，患者与对照组之间的卟啉和 ALA 浓度无显著差异。女性的尿卟啉浓度高于男性。
结论
燃烧煤炭暴露于 As 可能会影响卟啉生物合成。
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