单细胞凝胶电泳（SCG）——综述与讨论。
Single-cell gel electrophoresis (SCG)-A review and discussion.
机构信息
Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, 565, Osaka, Japan.
出版信息
Environ Health Prev Med. 1997 Jul;2(2):53-8. doi: 10.1007/BF02931964.
Single-cell gel electrophoresis (SCG) is a simple, sensitive and effective technique. Being able to reflect quantitatively the genotoxicity of many hazardous agents, it is promising for application in environmental genotoxic monitoring and the study of carcinogenesis. In clinics, it can be used to evaluate the DNA repair ability and monitor DNA breaks during cancer therapy. As a biomarker, it has its own merits and limitations, being different from other biomarkers such as sister chromatid exchange (SCE) test and micronuclei (MN) assay. In many studies, it is more sensitive than SCE or MN. Combination studies with other biomarkers like SCE, MN, chromosomal aberration, bcl-2 and genetic polymorphisms have begun to demonstrate its great importance for the understanding of carcinogenesis and the genotoxicities of environmental factors.
单细胞凝胶电泳（SCG）是一种简单、灵敏、有效的技术。它能够定量反映许多危险物质的遗传毒性，有望应用于环境遗传毒性监测和致癌作用研究。在临床上，它可用于评估 DNA 修复能力，并在癌症治疗过程中监测 DNA 断裂。作为一种生物标志物，它有其自身的优点和局限性，与姐妹染色单体交换（SCE）试验和微核（MN）测定等其他生物标志物不同。在许多研究中，它比 SCE 或 MN 更敏感。与 SCE、MN、染色体畸变、bcl-2 和遗传多态性等其他生物标志物的联合研究开始表明，它对理解致癌作用和环境因素的遗传毒性具有重要意义。
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