Department of Public Health, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, 470-11, Aichi, Japan.
Environ Health Prev Med. 1997 Oct;2(3):117-21. doi: 10.1007/BF02931976.
Immunocytotoxicity of beryllium (Be) was evaluated by studying cell viability, intracellular DNA synthesis and SRBC-IgM response in an in-vitro culture system using non-sensitized spleen cells of a C57BL mouse. Be addition showed a suppressive effect on cell viability, an enhancing effect on DNA synthesis and on IgM antibody production. The suppressive effect on cell viability manifested itself markedly as the concentration of Be was increased or the culture time was prolonged. The DNA synthesis-enhancing effect was noted at a relatively low concentration of Be (not more than 10μM). The enhancing effect on the IgM response was related to Be concentration at not more than 20μM. The experimental results mentioned above speculate that the cytotoxicity of Be shows a conflicting pattern of enhancement or suppression according to the concentration used and that immunologically it has a modulating effect or an activating effect on the immunocompetent cells.
采用体外培养体系，以未致敏的 C57BL 小鼠脾细胞为材料，研究了铍（Be）对细胞活力、细胞内 DNA 合成和 SRBC-IgM 反应的免疫细胞毒性。Be 对细胞活力具有抑制作用，对 DNA 合成和 IgM 抗体产生具有增强作用。随着 Be 浓度的增加或培养时间的延长，细胞活力的抑制作用明显。在相对较低的 Be 浓度（不超过 10μM）下即可观察到 DNA 合成的增强作用。对 IgM 反应的增强作用与不超过 20μM 的 Be 浓度有关。上述实验结果表明，根据所用浓度，Be 的细胞毒性表现出增强或抑制的矛盾模式，在免疫学方面，它对免疫活性细胞具有调节或激活作用。
