Department of Public Health, School of Medicine, Juntendo University, 2-1-1 Hongo, 113-8421, Bunkyo-ku, Tokyo, Japan.
Environ Health Prev Med. 2000 Jan;4(4):212-6. doi: 10.1007/BF02931260.
We investigated the relationship between the frequency of the common cold and the conditions of the body's immune system among members of a generally healthy population. Self-reporting questionnaires on the frequency of the common cold and on fatigue and stress conditions were administered to a total of 67 healthy individuals aged 22 to 50. The activities of natural killer (NK) cells, which were determined by Eu(3+)-DTPA release assay, and of NK cell members in the peripheral blood of the subjects were phenotypically (CD3 CD16(+) CD56(+)) analyzed with three-color flow cytometry. The results showed that the frequency of the common cold was significantly correlated with NK activity and NK subset (CD3(-)CD16(+) CD56(+)) frequency (r=-0.34 and-0.47 respectively, P<0.01). After adjusting for age, the mean NK subset (CD3(-)CD16(+)CD56) significandy differed (F=3.384, P<0.05) among the four frequency groups for the common cold, and the frequencies of the common cold were significandy different among the four stress/fatigue groups (F=8.016, P<0.001) for the males, as evaluated by ANCOVA. These results indicate that conditions of high stress and fatigue may increase the chance of catching the common cold due to a decrease in activities of NK cells.
我们研究了在一个普遍健康的人群中，普通感冒的频率与身体免疫系统状况之间的关系。我们向总共 67 名年龄在 22 岁至 50 岁之间的健康个体发放了关于普通感冒频率以及疲劳和压力状况的自我报告问卷。通过 Eu(3+)-DTPA 释放测定法测定自然杀伤 (NK) 细胞的活性，并用三色流式细胞术对受试者外周血中的 NK 细胞成员（CD3 CD16(+) CD56(+)）进行表型分析。结果表明，普通感冒的频率与 NK 活性和 NK 亚群（CD3(-)CD16(+) CD56(+)）频率呈显著负相关（r 分别为-0.34 和-0.47，P<0.01）。在调整年龄后，NK 亚群（CD3(-)CD16(+)CD56）的平均值在普通感冒的四个频率组之间存在显著差异（F=3.384，P<0.05），并且在男性中，普通感冒的频率在四个压力/疲劳组之间存在显著差异（F=8.016，P<0.001），这是通过协方差分析评估的。这些结果表明，高压力和疲劳的状态可能会由于 NK 细胞活性的降低而增加感染普通感冒的机会。
