Tabar A I, Sanz M L, Oehling A
Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Allergol Immunopathol (Madr). 1990 Jan-Feb;18(1):5-11.
After isolating the soluble factors and observing their biological activity of "in vitro" IgE synthesis, we decided to study the changes these molecules exercise on the expression of lymphocytes of IgE (FcR epsilon). We saw that the fractions from healthy subjects inhibited the expression of receptors quantified by immunofluorescence, reducing it to 15.5 and 15.75 (p less than 0.01). Three of the fractions from atopic individuals also inhibited this expression, with some average values of 13.75, 11.25 and 15.25, against 23 as the basal levels. Material from pollinic patients acted similarly, obtaining expression percentages of 13 and 14.5 (p less than 0.01). These findings, along with those obtained in the experiment of IgE synthesis, confirmed the presence of regulator factors in IgE synthesis in the 3 population groups, quantitatively different. These factors will be isotype specific and just as what happened in IgG synthesis, there was no modification in the expression of the mentioned immunoglobulin.
在分离出可溶性因子并观察其对“体外”IgE合成的生物学活性后，我们决定研究这些分子对IgE（FcRε）淋巴细胞表达的影响。我们发现，来自健康受试者的组分抑制了通过免疫荧光定量的受体表达，将其降至15.5和15.75（p小于0.01）。来自特应性个体的三个组分也抑制了这种表达，一些平均值为13.75、11.25和15.25，而基础水平为23。花粉症患者的材料表现类似，获得的表达百分比为13和14.5（p小于0.01）。这些发现，连同在IgE合成实验中获得的发现，证实了在这三个群体的IgE合成中存在调节因子，在数量上有所不同。这些因子将是同型特异性的，就像在IgG合成中发生的情况一样，上述免疫球蛋白的表达没有改变。
