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[Transgenic tomato plants expressing recA and NLS-recA-licBM3 genes as a model for studying meiotic recombination].

作者信息

Komakhin R A, Komakhina V V, Miliukova N A, Goldenkova-Pavlova I V, Fadina O A, Zhuchenko A A

出版信息

Genetika. 2010 Dec;46(12):1635-44.

Abstract

Homologous DNA recombination in eukaryotes is necessary to maintain genome stability and integrity and for correct chromosome segregation and formation of new haplotypes in meiosis. At the same time, genetic determination and nonrandomness of meiotic recombination restrict the introgression of genes and generation of unique genotypes. As one of the approaches to study and induce meiotic recombination in plants, it is recommended to use the recA gene of Escherichia coli. It is shown that the recA and NLS-recA-licBM3 genes have maternal inheritance and are expressed in the progeny of transgenic tomato plants. Plants expressing recA or NLS-recA-licBM3 and containing one T-DNA insertion do not differ in pollen fertility from original nontransgenic forms and can therefore be used for comparative studies of the effect of bacterial recombinases on meiotic recombination between linked genes.

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