Maĭkova O O, Itskovich V B, Semiturkina N A, Kaliuzhnaia O V, Belikov S I
Genetika. 2010 Dec;46(12):1670-7.
Morphological and molecular genetic data for freshwater sponges from the lakes of Tuva Depression, Baikalospongia dzhegatajensis (Rezvo, 1936), forms Dzh05 and Dzh06, from Chagatai Lake, as well as forms TKhl and TKh2, from the Lake Tore-Khol, were obtained and examined. In the sponges examined, which on phylogenetic tree clustered together with the Ephydatia fluviatilis (Linneaus, 1758) sponge from the family Spongillidae, the ITS rDNA regions were sequenced. Comparison of highly variable interal spacer regions of the mitochondrial genome was performed using corresponding sequences of three sponges from the family Spongillidae (E. fluviatilis, E. muelleri and Spongilla lacustris), sponges from the Chagatai and Tore-Khol lakes (Dzh06 and TKh2) with an unknown status, and sponges from the Baikalian family Lubomirskiidae. Minimum genetic differences were observed between E. fluviatilis, Dzh06, and TKh2 (from 0.003 to 0.01% of nucleotide substitutions), while maximum differences were found between the species of Lubomirskiidae and Spongillidae (from 0.928 to 2.06%). The data obtained indicated that sponges from Chagatai and Tore-Khol lakes were most close to E. fluviatilis.
获取并研究了来自图瓦洼地湖泊的淡水海绵、恰加泰湖的哲加泰贝加尔海绵(Rezvo,1936年)的Dzh05和Dzh06形态以及分子遗传学数据,以及托雷 - 霍尔湖的TKhl和TKh2形态的数据。在所研究的海绵中,其在系统发育树上与海绵科的弗氏淡水海绵(Linneaus,1758年)聚类在一起,对其ITS rDNA区域进行了测序。使用海绵科的三种海绵(弗氏淡水海绵、米勒淡水海绵和湖栖海绵)、恰加泰湖和托雷 - 霍尔湖状态未知的海绵(Dzh06和TKh2)以及贝加尔湖的卢博米尔希海绵科海绵的相应序列,对线粒体基因组高度可变的内部间隔区进行了比较。在弗氏淡水海绵、Dzh06和TKh2之间观察到最小的遗传差异(核苷酸替换率为0.003%至0.01%),而在卢博米尔希海绵科和海绵科的物种之间发现了最大差异(从0.�28%至2.06%)。所获得的数据表明,恰加泰湖和托雷 - 霍尔湖的海绵与弗氏淡水海绵最为接近。