Shu Xin-Ying, Meng Xian-Sheng, Pan Ying, Han Ling, Bao Yong-Rui, Guo Xiao-Rui
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
Zhong Yao Cai. 2010 Nov;33(11):1788-91.
To study the purification technology of TGP from Paeonia lactiflora by ethanol gradient combined with resin processing and determine the optimum technological conditions and parameters.
Using orthogonal test design to investigate the effect of ethanol gradient treatment on the content of TGP. Moreover, from the static and dynamic adsorption nine types of macroporous adsorption resin were evaluated to select the best resin type and the optimum separation and purification conditions.
The best technology of Paeonia lactiflora ethanol precipitation was concentration of 1 g crude drug/mL precipitated by 95% ethanol to 90% concentration and then frozen for 10 h. HPD300 resin was the optimal model for the separation and purification of TGP from Paeonia lactiflora, with 5BV of 50% ethanol eluenting and the ratio of herb to resin was 2:1 .
This technology is suitable and advanced for industry production and it is simple and convenient, rapid, accurate, etc.
研究采用乙醇梯度结合树脂处理从芍药中纯化芍药苷的工艺，确定最佳工艺条件和参数。
采用正交试验设计考察乙醇梯度处理对芍药苷含量的影响。此外，通过静态和动态吸附对9种大孔吸附树脂进行评价，以筛选出最佳树脂类型及最佳分离纯化条件。
芍药乙醇沉淀的最佳工艺为：将1 g生药/mL的药液用95%乙醇沉淀至浓度为90%，然后冷冻10 h。HPD300树脂是从芍药中分离纯化芍药苷的最佳型号，用50%乙醇以5BV进行洗脱，药材与树脂比例为2:1。
该工艺适用于工业化生产，具有简单方便、快速准确等优点，且先进可行。
