[Studies on purification of total glycosides of paeony (TGP) from Paeonia lactiflora by ethanol gradient combined with resin processing].

OBJECTIVE

To study the purification technology of TGP from Paeonia lactiflora by ethanol gradient combined with resin processing and determine the optimum technological conditions and parameters.

METHODS

Using orthogonal test design to investigate the effect of ethanol gradient treatment on the content of TGP. Moreover, from the static and dynamic adsorption nine types of macroporous adsorption resin were evaluated to select the best resin type and the optimum separation and purification conditions.

RESULTS

The best technology of Paeonia lactiflora ethanol precipitation was concentration of 1 g crude drug/mL precipitated by 95% ethanol to 90% concentration and then frozen for 10 h. HPD300 resin was the optimal model for the separation and purification of TGP from Paeonia lactiflora, with 5BV of 50% ethanol eluenting and the ratio of herb to resin was 2:1 .

CONCLUSION

This technology is suitable and advanced for industry production and it is simple and convenient, rapid, accurate, etc.