大孔吸附树脂分离纯化连翘叶中苯乙醇苷类成分的研究
[Studies on separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin].
作者信息
Jing Hui, Li Jiao-She, Yang Yin-Jing
机构信息
Department of Pharmacy, Xi'an Medical College, Xi'an 710021, China.
出版信息
Zhong Yao Cai. 2010 Sep;33(9):1487-90.
OBJECTIVE
To study the technology for separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin.
METHODS
The absorption and separation abilities of 10 kinds of adsorption resin were studied and the separation and purification technological process of phenylethanoid glycosides from leaves of Forsythia suspensa was investigated by HPLC with the content of Forsythoside A as an index.
RESULTS
The optimal conditions were as follows: taking AB-8 resin column as adsorbent, the resin was washed by 20BV distilled water to remove impurity and 8BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2BV/h, the content of Forsythoside A in the extract was 34.8%.
CONCLUSION
The process with AB-8 resin is an effective method to separate and purify phenylethanoid glycosides.
目的
研究大孔吸附树脂分离纯化连翘叶中苯乙醇苷类成分的工艺。
方法
考察10种吸附树脂的吸附分离性能，以连翘苷A含量为指标，采用高效液相色谱法研究连翘叶中苯乙醇苷类成分的分离纯化工艺。
结果
最佳工艺条件为：以AB-8型树脂柱为吸附剂，先用20倍柱体积蒸馏水冲洗树脂以除去杂质，再用8倍柱体积30%乙醇洗脱苯乙醇苷类成分，洗脱流速为2倍柱体积/小时，提取物中连翘苷A含量为34.8%。
结论
AB-8树脂法是分离纯化苯乙醇苷类成分的有效方法。
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