Wang Zhi-Ping, Gao Ying, Li Wei-Min, Liu Jie
School of Chinese materia medica, Guangzhou University of TCM, Guangzhou 510006, China.
Zhong Yao Cai. 2010 Jul;33(7):1163-6.
Through systematic study on many factors of influence of macroporous resin adsorption and separation, screening the best macroporous resin for purification of total flavonoids and saponins of Astragalus.
The adsorption and desorption properties of total flavonoids and saponins on macroporous resins including D101, AB-8, XAD-1, HP-20, SP825, LSA-40, LX-18, XDA-5 and ADS-7 were compared. According to the results, XDA-5, D101 and AB-8 offered higher adsorption and desorption capacities and higher adsorption speed for total flavonoids and saponins than other resins. Column packed with XDA-5 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process.
XDA-5 type macroporous resin owned optimum adsorption and elution parameters. The dynamic adsorption ratio of total flavonoids of Astragalus was 58.83 mg/mL after eluted with 4BV of distilled water and 4BV of 30%, 50%, 70% ethanol, respectively. Contents of total flavonoids and saponins of Astragalus were 28.75%, 44.38%, respectively.
It is a reliable and accurate method for development and industrial production of compound drug containing Astragalus.
通过对大孔树脂吸附分离多种影响因素的系统研究，筛选出用于纯化黄芪总黄酮和皂苷的最佳大孔树脂。
比较了D101、AB - 8、XAD - 1、HP - 20、SP825、LSA - 40、LX - 18、XDA - 5和ADS - 7等大孔树脂对总黄酮和皂苷的吸附和解吸性能。结果显示，XDA - 5、D101和AB - 8对总黄酮和皂苷的吸附和解吸能力以及吸附速度均高于其他树脂。采用装有XDA - 5树脂的柱进行动态吸附和解吸试验，以优化分离工艺。
XDA - 5型大孔树脂具有最佳的吸附和洗脱参数。用4BV蒸馏水及4BV的30%、50%、70%乙醇洗脱后，黄芪总黄酮的动态吸附量为58.83mg/mL。黄芪总黄酮和皂苷的含量分别为28.75%、44.38%。
该方法对于含黄芪复方药物的开发及工业化生产是一种可靠且准确的方法。
