Kakpakova E S, Massino Iu S
Genetika. 1978 Nov;14(11):2025-8.
New biochemically marked Djungarian hamster cell line (DX-TK-) was established. These cells are resistant to 5-bromodeoxyuridine (25 mkg/ml) and deficient in thymidine kinase activity (TK-). Due to this biochemical defect they have lost the ability to grow in HAT medium. DX-TK- cells are malignant. They grow as tumours after the inoculation to newborn Djungarian hamsters. Tumorigenecity of DX-TK- cells was decreased as compared with the parent TK+ cell line. DX-TK- cell line is a hypodiploid cell culture (26 chromosomes) with 7 chromosome markers easily identified by means of G-band staining. This line is a new model for somatic cell genetic experiments, particularly for somatic cell hybridization.
建立了新的生化标记的黑线仓鼠细胞系（DX-TK-）。这些细胞对5-溴脱氧尿苷（25微克/毫升）具有抗性，且胸苷激酶活性缺乏（TK-）。由于这种生化缺陷，它们失去了在HAT培养基中生长的能力。DX-TK-细胞具有恶性。接种到新生黑线仓鼠后会形成肿瘤。与亲本TK+细胞系相比，DX-TK-细胞的致瘤性降低。DX-TK-细胞系是一种亚二倍体细胞培养物（26条染色体），有7个染色体标记，通过G带染色很容易识别。该细胞系是体细胞遗传实验，特别是体细胞杂交的新模型。
