Malakhova E M, Kakpakova E S
Genetika. 1984 Jul;20(7):1155-63.
Tumor Djungarian hamster cells resistant to 5-bromodeoxyuridine (5-BrdU) were inoculated to newborn hamsters. Tumors occurred in animals and were seeded into HAT medium in vitro. This procedure permitted to select hybrids between tumor and normal cells established in vivo. Hybrid nature of cell cultures was confirmed by karyological analysis. Hybrid cells were tested for their ability to grow progressively in newborn Djungarian hamsters and to form colonies in soft agar. The hybrid cells were less malignant than 5-BrdU-resistant tumor cells, but they could grow in soft agar with the efficiency of the parental tumor cells. Chromosomal constitution of the hybrid tumors indicate that as a rule, the expression of malignancy correlates with elimination of the morphologically normal chromosome pairs No 1, 4, 6, 8. Our data suggest that at least two genes located on different chromosomes of the normal cell are needed for suppression of malignancy in somatic cell hybrids.
将对5-溴脱氧尿苷（5-BrdU）具有抗性的肿瘤黑线仓鼠细胞接种到新生仓鼠体内。动物体内出现肿瘤，并将其接种到体外的HAT培养基中。该程序允许选择在体内建立的肿瘤细胞与正常细胞之间的杂种。通过染色体分析证实了细胞培养物的杂种性质。测试杂种细胞在新生黑线仓鼠体内进行渐进性生长以及在软琼脂中形成集落的能力。杂种细胞的恶性程度低于对5-BrdU具有抗性的肿瘤细胞，但它们在软琼脂中生长的效率与亲代肿瘤细胞相同。杂种肿瘤的染色体组成表明，通常情况下，恶性程度的表达与形态学上正常的第1、4、6、8号染色体对的消除相关。我们的数据表明，体细胞杂种中至少需要位于正常细胞不同染色体上的两个基因来抑制恶性程度。
