Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
PLoS One. 2011 Mar 17;6(3):e17972. doi: 10.1371/journal.pone.0017972.
The etiology of anti-neutrophil cytoplasmic antibodies (ANCA) associated vasculitides (AAV) is unknown, but the association between infections and autoimmunity has been studied extensively. In 2004, a novel theory was proposed that could link infection and autoimmunity. This 'theory of autoantigen complementarity' was based on the serendipitous finding of antibodies against complementary-PR3 (cPR3) in patients with PR3-ANCA-associated vasculitis. cPR3 demonstrated homology to several bacterial proteins, and it was hypothesized that PR3-ANCA develop in response to anti-cPR3 antibodies, as a consequence of the anti-idiotypic network. These data have not been confirmed in other patient cohorts. We investigated the presence of anti-cPR3 antibodies in a Dutch cohort of PR3-ANCA-associated vasculitis patients. Anti-cPR3 reactivity was determined in serum using ELISA. Two separate batches of cPR3 were used to determine reactivity in two separate cohorts of PR3-ANCA-associated vasculitis patients. We found that anti-cPR3-reactivity was not increased in our PR3-ANCA-associated vasculitis patients, in comparison to control groups. Further research will be necessary to prove the concept of autoantigen complementarity in autoimmune diseases.
抗中性粒细胞胞浆抗体（ANCA）相关性血管炎（AAV）的病因尚不清楚，但感染与自身免疫之间的关系已得到广泛研究。2004 年，提出了一种新的理论，该理论可以将感染和自身免疫联系起来。这种“自身抗原互补理论”基于对 PR3-ANCA 相关性血管炎患者中针对互补-PR3（cPR3）的抗体的偶然发现。cPR3 与几种细菌蛋白具有同源性，据推测，PR3-ANCA 是针对抗 cPR3 抗体产生的，是抗独特型网络的结果。这些数据尚未在其他患者队列中得到证实。我们在荷兰 PR3-ANCA 相关性血管炎患者队列中研究了抗 cPR3 抗体的存在。使用 ELISA 法在血清中测定抗 cPR3 反应性。使用两批不同的 cPR3 来确定两批 PR3-ANCA 相关性血管炎患者队列中的反应性。我们发现，与对照组相比，PR3-ANCA 相关性血管炎患者的抗 cPR3 反应性没有增加。需要进一步的研究来证明自身抗原互补性在自身免疫性疾病中的概念。
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