Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-13 Nagatsuta, Yokohama 226-8503, Japan.
Chemistry. 2011 May 2;17(19):5344-9. doi: 10.1002/chem.201003285. Epub 2011 Mar 24.
A fluorometric sensor for detection and identification of biogenic amines with carboxylic acid modified tetraphenylethenes (TPEs) based on aggregation-induced emission (AIE) is reported. A mixture of the carboxylic acid substituted TPE and biogenic amines displayed a blue emission on aggregation, which serves as a "turn-on" fluorescent sensor for the amines, the degree of fluorescence enhancement being dependent on the amine. The chromic responses were utilized to distinguish the amines. A fluorometric sensor array of three TPEs with carboxylic acid groups was shown to identify accurately 10 different amines, including biogenic amines. The response patterns were systematically classified by using linear discriminant analysis (LDA) with 98% classification accuracy. Additional information on the concentration of histamine in a "tuna fish matrix" as an example was assessed by the further analysis of the fluorescence intensity, demonstrating a test for food freshness and quality.
报道了一种基于聚集诱导发射（AIE）的羧酸修饰四苯乙烯（TPE）的用于检测和识别生物胺的荧光传感器。羧酸取代的 TPE 与生物胺的混合物在聚集时显示出蓝色发射，这可用作胺的“开启”荧光传感器，荧光增强的程度取决于胺。颜色响应可用于区分胺。带有羧酸基团的三个 TPE 的荧光传感器阵列可准确识别 10 种不同的胺，包括生物胺。使用线性判别分析（LDA）对响应模式进行系统分类，分类准确率为 98%。通过进一步分析荧光强度评估了“金枪鱼鱼基质”中组氨酸浓度的其他信息，证明了该方法可用于测试食品的新鲜度和质量。
