基于封端二氧化硅颗粒的比色化学计量计用于检测精胺和亚精胺。
Chromogenic Chemodosimeter Based on Capped Silica Particles to Detect Spermine and Spermidine.
作者信息
Barros Mariana, López-Carrasco Alejandro, Amorós Pedro, Gil Salvador, Gaviña Pablo, Parra Margarita, El Haskouri Jamal, Terencio Maria Carmen, Costero Ana M
机构信息
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitad Politècnica de València, Universitat de València, Doctor Moliner 50, Burjassot, 46100 Valencia, Spain.
Instituto de Ciencia de Materiales (ICMUV), Universitat de València, P.O. Box 2085, 46071 Valencia, Spain.
出版信息
Nanomaterials (Basel). 2021 Mar 23;11(3):818. doi: 10.3390/nano11030818.
A new hybrid organic-inorganic material for sensing spermine (Spm) and spermidine (Spd) has been prepared and characterized. The material is based on MCM-41 particles functionalized with an N-hydroxysuccinimide derivative and loaded with Rhodamine 6G. The cargo is kept inside the porous material due to the formation of a double layer of organic matter. The inner layer is covalently bound to the silica particles, while the external layer is formed through hydrogen and hydrophobic interactions. The limits of detection determined by fluorimetric titration are 27 µM and 45 µM for Spm and Spd, respectively. The sensor remains silent in the presence of other biologically important amines and is able to detect Spm and Spd in both aqueous solution and cells.
一种用于检测精胺（Spm）和亚精胺（Spd）的新型有机-无机杂化材料已被制备并表征。该材料基于用N-羟基琥珀酰亚胺衍生物功能化并负载罗丹明6G的MCM-41颗粒。由于形成了一层有机物双层，货物被保留在多孔材料内部。内层与二氧化硅颗粒共价结合，而外层则通过氢键和疏水相互作用形成。通过荧光滴定法测定的Spm和Spd的检测限分别为27 μM和45 μM。该传感器在其他生物学上重要的胺存在时保持沉默，并且能够在水溶液和细胞中检测Spm和Spd。
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