通过还原胺化和酰肼偶联对生色团标记的寡糖进行紫外光解。

Ultraviolet photodissociation of chromophore-labeled oligosaccharides via reductive amination and hydrazide conjugation.

机构信息

Department Chemical Engineering, The University of Texas at Austin, 1 University Station A5300, Texas 78712, USA.

出版信息

J Mass Spectrom. 2011 Apr;46(4):359-66. doi: 10.1002/jms.1901.

DOI:10.1002/jms.1901

Abstract

The fragmentation patterns of hydrazide-conjugated and reductively aminated oligosaccharides, including lacto-N-fucopentaoses and lacto-N-difucohexaoses, produced on collisionally induced dissociation (CID) and ultraviolet photodissociation (UVPD) in a quadrupole ion trap are presented. The two derivatization methods generate different cross-ring cleavages on UVPD and CID. UVPD of hydrazide-conjugated oligosaccharides yield predominant (2, 4)A-type cross-ring cleavage ions. In contrast, UVPD of aminated oligosaccharides results mainly in (0, 1)A-type ions. Moreover, more extensive dual-cleavage pathways (i.e. internal fragment ions) were observed on UVPD.

摘要

本文介绍了在四极离子阱中通过碰撞诱导解离（CID）和紫外光解（UVPD）产生的酰肼偶联和还原胺化寡糖（包括乳-N-岩藻五糖和乳-N-二岩藻六糖）的碎片模式。这两种衍生化方法在 UVPD 和 CID 上产生不同的跨环裂解。酰肼偶联寡糖的 UVPD 产生主要的（2,4）A 型跨环裂解离子。相比之下，氨基化寡糖的 UVPD 主要产生（0,1）A 型离子。此外，在 UVPD 上观察到更多的双裂解途径（即内部片段离子）。