Peter M G, Schweikart F
Institut für Organische Chemie und Biochemie, Rheinischen Friedrich-Wilhelms-Universität Bonn.
Biol Chem Hoppe Seyler. 1990 Jun;371(6):471-3. doi: 10.1515/bchm3.1990.371.1.471.
Membrane preparations of Artemia salina synthetize radiolabelled chitin from UDP-[U-14C]GlcNAc at a low rate (Horst, M.N. (1981) J. Biol. Chem. 256, 1412-1419). We now report that, when the specific endochitinase inhibitor allosamidin is present in addition to the established activators trypsin and GlcNAc, incorporation of [U-14C]GlcNAc into chitin is increased up to 58-fold over the basic synthesis rate. Thus, a greatly enhanced apparent chitin synthase activity is observed in membranes from an arthropod species when simultaneous degradation of chitin is inhibited.
卤虫的膜制剂以低速率从UDP-[U-¹⁴C]GlcNAc合成放射性标记的几丁质（霍斯特，M.N.（1981年）《生物化学杂志》256卷，1412 - 1419页）。我们现在报告，当除了既定的激活剂胰蛋白酶和GlcNAc之外还存在特异性内切几丁质酶抑制剂阿洛沙米丁时，[U-¹⁴C]GlcNAc掺入几丁质的量比基础合成速率提高了58倍。因此，当几丁质的同时降解受到抑制时，在一种节肢动物物种的膜中观察到明显增强的几丁质合酶活性。
