吉氏盘尾丝虫雌虫中的几丁质酶及其被脲基壳聚糖苷的抑制作用

Chitinase in female Onchocerca gibsoni and its inhibition by allosamidin.

作者信息

Gooday G W, Brydon L J, Chappell L H

机构信息

Department of Genetics and Microbiology, University of Aberdeen, U.K.

出版信息

Mol Biochem Parasitol. 1988 Jun;29(2-3):223-5. doi: 10.1016/0166-6851(88)90077-1.

DOI:10.1016/0166-6851(88)90077-1

PMID:3412376

Abstract

Chitinase activity has been detected in female worms of Onchocerca gibsoni. With 3,4-dinitrophenyl-tetra-N-acetylchitotetraoside as substrate 50% of maximum activity was achieved at about 25 microM substrate, with inhibition above 50 microM substrate. The antibiotic allosamidin very strongly inhibited the chitinase activity, 50% inhibition being achieved by 200 pM allosamidin in the presence of 45 microM substrate.

摘要

在吉氏盘尾丝虫的雌虫中检测到了几丁质酶活性。以3,4 - 二硝基苯基 - 四 - N - 乙酰壳四糖为底物，在约25微摩尔底物浓度时达到最大活性的50%，底物浓度高于50微摩尔时活性受到抑制。抗生素别洛沙米丁能非常强烈地抑制几丁质酶活性，在存在45微摩尔底物的情况下，200皮摩尔别洛沙米丁可实现50%的抑制率。