Department of Otolaryngology, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Cancer Lett. 2011 Jul 1;306(1):60-6. doi: 10.1016/j.canlet.2011.02.032. Epub 2011 Mar 24.
The aim of this study was to investigate the radiosensitizing potential of D-allose in human head and neck cancer cells. HSC-3 cells were treated with or without D-allose for 6 h and then irradiated (2-6 Gy). The combination of D-allose and radiation was more effective than either agent alone. The radiation enhancement ratios at the 37% survival level were 1.61 and 2.11 for 10 mM and 25 mM D-allose treatment, respectively. The combination of D-allose and radiation also reduced the cell proliferation in 3D culture experiments. Although the mRNA expression of TXNIP was not increased by radiation alone, combined use with D-allose markedly elevated TXNIP expression. The combination of D-allose and radiation significantly induced intracellular reactive oxygen species (ROS) and apoptosis compared to that induced by either agent alone. This study shows that D-allose enhances the effect of radiation, suggesting a potential clinical application of combination treatment with D-allose and radiation for head and neck cancer.
本研究旨在探讨 D-allo 果糖在人头颈癌细胞中的放射增敏作用。将 HSC-3 细胞用或不用 D-allo 果糖处理 6 小时，然后进行照射（2-6 Gy）。D-allo 果糖与辐射的联合作用比单独使用任何一种药物都更有效。在 37%存活水平下，10 mM 和 25 mM D-allo 果糖处理的放射增敏比分别为 1.61 和 2.11。D-allo 果糖和辐射的联合作用还减少了 3D 培养实验中的细胞增殖。尽管单独使用辐射不会增加 TXNIP 的 mRNA 表达，但与 D-allo 果糖联合使用显著增加了 TXNIP 的表达。与单独使用任何一种药物相比，D-allo 果糖和辐射的联合作用显著诱导了细胞内活性氧（ROS）和细胞凋亡。本研究表明，D-allo 果糖增强了辐射的效果，提示 D-allo 果糖和辐射联合治疗头颈部癌症具有潜在的临床应用价值。
