Badial Peres R, Oliveira-Filho José P, Cunha Paulo Henrique J, Araújo João P, Peiró Juliana R, Divers Thomas J, Winand Nena J, Borges Alexandre S
Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, Univ Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Vet Immunol Immunopathol. 2011 May 15;141(1-2):157-61. doi: 10.1016/j.vetimm.2011.02.009. Epub 2011 Feb 23.
Hepcidin is a highly conserved disulfide-bonded peptide that plays a central role in iron homeostasis. During systemic inflammation, hepcidin up-regulation is responsible for hypoferremia. This study aimed to analyze the influence of the inflammatory process induced by complete Freund's adjuvant (CFA) or lipopolysaccharide (LPS) on the liver expression of hepcidin mRNA transcripts and plasma iron concentration of sheep. The expression levels of hepcidin transcripts were up-regulated after CFA or LPS. Hypoferremic response was observed at 12 h (15.46 ± 6.05 μmol/L) or 6h (14.59 ± 4.38μmol/L) and iron reached its lowest level at 96 h (3.08 ± 1.18 μmol/L) or 16h (4.06 ± 1.58 μmol/L) after CFA administration or LPS infusion, respectively. This study demonstrated that the iron regulatory hormone hepcidin was up-regulated in sheep liver in response to systemic inflammation. These findings extend our knowledge on the relationship between the systemic inflammatory response, hepcidin and iron, and provide a starting point for additional studies on iron metabolism and the inflammatory process in sheep.
铁调素是一种高度保守的二硫键连接的肽，在铁稳态中起核心作用。在全身炎症期间，铁调素上调是低铁血症的原因。本研究旨在分析完全弗氏佐剂（CFA）或脂多糖（LPS）诱导的炎症过程对绵羊肝脏中铁调素mRNA转录物表达和血浆铁浓度的影响。CFA或LPS后铁调素转录物的表达水平上调。分别在给予CFA或输注LPS后12小时（15.46±6.05μmol/L）或6小时（14.59±4.38μmol/L）观察到低铁血症反应，铁在96小时（3.08±1.18μmol/L）或16小时（4.06±1.58μmol/L）达到最低水平。本研究表明，在全身炎症反应中，绵羊肝脏中的铁调节激素铁调素上调。这些发现扩展了我们对全身炎症反应、铁调素和铁之间关系的认识，并为进一步研究绵羊铁代谢和炎症过程提供了一个起点。
