Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Av. Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, 79070-900, Brazil.
Anim Reprod Sci. 2011 May;125(1-4):165-9. doi: 10.1016/j.anireprosci.2011.02.030. Epub 2011 Mar 9.
The aim of this research was to evaluate the intrinsic rate of spermatogenesis in adult free-ranging feral pigs. Twelve adult male free-ranging feral pigs were captured, sedated, and orchidectomized, and then were released and observed to complete recovery and return to their natural environment. Fragments of the testes were embedded in plastic resin and used to prepare slides for histometric analysis. Characteristics investigated included cell populations in the seminiferous epithelium in stage 1 of the cycle of the seminiferous epithelium, intrinsic rate of spermatogenesis and Sertoli cell index. The efficiency coefficient of spermatogonial mitosis was 7.59, the meiotic index was 3.03, the overall yield of spermatogenesis was 23.97 and the cell loss ratio during the meiotic prophase was 1.04. Each Sertoli cell supported an average of 0.92 type A spermatogonia, 7.01 primary spermatocytes in preleptotene/leptotene, 7.30 primary spermatocytes in pachytene and 22.16 round spermatids. In conclusion, the results of the present study indicate that the supporting capacity of Sertoli cells in free-ranging feral pigs is among the greatest values reported for most domestic animals, and the overall yield of spermatogenesis is comparable to that reported in wild boars.
本研究旨在评估成年自由放养野猪的精子发生固有率。 12 只成年雄性自由放养野猪被捕获、镇静并进行睾丸切除术，然后被释放并观察其完全恢复并返回其自然环境。睾丸的片段被嵌入塑料树脂中，用于制备用于组织学分析的幻灯片。研究的特征包括处于生精上皮周期 1 期的生精上皮中的细胞群体、精子发生固有率和支持细胞指数。精原细胞有丝分裂的效率系数为 7.59，减数分裂指数为 3.03，精子发生的总产率为 23.97，减数分裂前期的细胞丢失率为 1.04。每个支持细胞平均支持 0.92 个 A 型精原细胞、7.01 个preleptotene/leptotene 的初级精母细胞、7.30 个 pachytene 的初级精母细胞和 22.16 个圆形精子细胞。 总之，本研究的结果表明，自由放养野猪的支持细胞的支持能力是大多数家畜中报告的最高值之一，并且精子发生的总产率与野猪中报告的相当。
