General Biology Department, Federal University of Viçosa, Minas Gerais 36570-000, Brazil.
Anim Reprod Sci. 2011 Sep;127(3-4):202-12. doi: 10.1016/j.anireprosci.2011.07.016. Epub 2011 Aug 16.
Information on the reproductive biology of neotropical cervids is scarce. Therefore, the aim of this study was to perform biometric, histologic and stereologic analyses of the brown brocket deer Mazama gouazoubira testis, with an emphasis on the intrinsic yield and the Sertoli cell index. Seven adult males kept in captivity were used. The animals were immobilized; anesthetized and testicle fragments were obtained by biopsy incision. The material was fixed, processed and examined by routine histological methods for light microscopy. The average body weight was 17.2kg, from which 0.40% were allocated in gonads and 0.33% in seminiferous tubules, which represented 85.9% of the testis parenchyma. The mean albuginea width and volume were 345.7μm and 3.5mL (5.3% of the testicular weight), respectively. The mean mediastinum volume of both testicles was 1.0mL (1.5% of the testicular weight) and the testicular parenchyma volume corresponded to 93.1% of total testicular weight (64.9g). The seminiferous tubules diameter was 224.4μm, while the epithelium height was 69.6μm. On average, an adult brown brocket deer showed a total of 1418m of seminiferous tubules in both testicles (21.5m per gram of testis). Each stage I seminiferous tubular cross section contained 1.10 type A spermatogonia, 13.4 primary spermatocytes in pre-leptotene/leptotene, 13.7 spermatocytes in pachytene, 48.8 round spermatids and 3.7 Sertoli cells. The general yield of spermatogenesis was 44.7 cells and the Sertoli cell index was 13.2. The qualitative and quantitative description of testicular histology of brown brocket deer help to understand its spermatogenic process and to establish parameters for the reproductive biology of this wild species. Furthermore, the data from the present research will help further studies using other species of Brazilian cervids, especially endangered ones, making an additional effort to the species preservation.
有关新热带鹿类生殖生物学的信息很少。因此，本研究的目的是对棕马鹿 Mazama gouazoubira 的睾丸进行生物测量、组织学和体视学分析，重点是内在产量和支持细胞指数。使用了 7 只圈养的成年雄性动物。动物被固定、麻醉，并通过活检切口获得睾丸碎片。采用常规组织学方法对材料进行固定、处理和检查，以便进行光镜检查。平均体重为 17.2kg，其中 0.40%分配给性腺，0.33%分配给曲细精管，占睾丸实质的 85.9%。平均白膜宽度和体积分别为 345.7μm 和 3.5mL（占睾丸重量的 5.3%）。两个睾丸的中隔体积平均为 1.0mL（占睾丸重量的 1.5%），睾丸实质体积占睾丸总重量的 93.1%（64.9g）。曲细精管直径为 224.4μm，而上皮高度为 69.6μm。平均而言，一只成年棕马鹿的两个睾丸的曲细精管总长度为 1418m（每克睾丸 21.5m）。每个 I 期生精小管横截面包含 1.10 个 A 型精原细胞、13.4 个 pre-leptotene/leptotene 初级精母细胞、13.7 个粗线期精母细胞、48.8 个圆形精子细胞和 3.7 个支持细胞。精子发生的总产量为 44.7 个细胞，支持细胞指数为 13.2。棕马鹿睾丸组织学的定性和定量描述有助于了解其精子发生过程，并为该野生动物的生殖生物学建立参数。此外，本研究的数据将有助于进一步研究巴西其他鹿类物种，特别是濒危物种，为物种保护做出额外努力。
