Walker M C, Povey S, Parrington J M, Riddle P N, Knuechel R, Masters J R
Cancer Research Campaign, London, U.K.
Eur J Cancer. 1990;26(6):742-7. doi: 10.1016/0277-5379(90)90133-e.
Cisplatin-resistant cells were derived in vitro from a human bladder carcinoma cell line (RT112) and a testicular tumour cell line (SuSa) by continuous exposure to increasing concentrations of cisplatin for 14 and 11 months, respectively. Both resistant cell lines had a four-fold level of resistance relative to their parental cell lines, comparing the cisplatin concentration to inhibit colony forming ability by 70%. These levels of resistance were retained in the absence of cisplatin for at least 3 months. In each case, four-fold fewer micronuclei were produced in the resistant lines by the same cisplatin concentrations. Cross-resistance to carboplatin and methotrexate was observed in both resistant cell lines, but neither line was resistant to doxorubicin. Isozyme and DNA analysis with hypervariable probes confirmed the origin of each resistant cell line from its parental line. Population doubling times and intermitotic times were similar in each of the pairs of cell lines. Karyotyping showed that the resistant cell lines had gained and lost marker chromosomes, but there were no changes common to both resistant cell lines.
顺铂耐药细胞系分别通过将人膀胱癌细胞系（RT112）和睾丸肿瘤细胞系（SuSa）连续暴露于浓度递增的顺铂中14个月和11个月，在体外获得。与亲代细胞系相比，两种耐药细胞系在将顺铂浓度抑制集落形成能力达70%时，均具有四倍水平的耐药性。在无顺铂的情况下，这些耐药水平至少维持3个月。在每种情况下，相同顺铂浓度在耐药细胞系中产生的微核数量减少了四倍。在两种耐药细胞系中均观察到对卡铂和甲氨蝶呤的交叉耐药，但两种细胞系对阿霉素均不耐药。使用高变探针进行的同工酶和DNA分析证实了每个耐药细胞系均源自其亲代细胞系。每组细胞系中的群体倍增时间和有丝分裂间期时间相似。核型分析表明，耐药细胞系有标记染色体的增减，但两种耐药细胞系没有共同的变化。
