University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, Slovakia.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 May;28(5):608-18. doi: 10.1080/19440049.2011.561879.
Maduramycin is a coccidiostat authorized as a feed additive in poultry. Council Directive 96/23/EC stipulates that monitoring of foods of animal origin for residues of coccidiostats is mandatory. The aim of the present study was to evaluate the STAR for the screening of maduramycin residues in the tissues of broiler chickens and pheasants. Both animal species were supplied feed medicated with Cygro l% premix according to recommendations for use (5 mg kg(-1) of complete feed). The residues were investigated for a period of 7 days: day 0 (the last day of the administration of maduramycin), days 1-5 (the days of the withdrawal period) and day 6 (the first day after elapse of the withdrawal period). According to STAR the positivity of the sample (the presence of residues of antibacterial substances) is indicated by a zone of inhibition exceeding 2 or 4 mm in width, depending on the test organism. Maduramycin residues were detected only on the plates seeded with the test organism Bacillus stearothermophilus var. calidolactis ATCC 10149. The results showed that there was higher potential for the presence of maduramycin residues in broiler chickens than in pheasants. All chicken tissues (muscle/thigh and breast/gizzard, liver, heart, kidneys, spleen, lungs) were positive for maduramycin (inhibition zones ≥4 mm) not only throughout the withdrawal period, but also even 5 days after elapse of the withdrawal period. In the case of pheasants the positive results were detected in the gizzard, liver, heart, kidneys, spleen and lungs. On day 5 of the withdrawal period no positive results were detected; however, on day 6 the heart and spleen were positive again.
马杜霉素是一种被批准作为家禽饲料添加剂的抗球虫药。委员会指令 96/23/EC 规定，必须监测动物源性食品中的抗球虫药残留。本研究的目的是评估 STAR 用于筛选肉鸡和雉鸡组织中的马杜霉素残留。这两个动物物种都按照推荐使用量（每吨配合饲料中添加 5 毫克）用 Cygro l%预混料添加药物喂养。残留调查持续 7 天：第 0 天（马杜霉素给药的最后一天）、第 1-5 天（停药期）和第 6 天（停药期结束后的第一天）。根据 STAR，如果样品存在抑制区宽度超过 2 或 4 毫米的抑菌带，则表示样品呈阳性（存在抗菌物质残留），具体取决于测试生物体。仅在用测试生物体嗜热脂肪芽孢杆菌 var. calidolactis ATCC 10149 接种的平板上检测到马杜霉素残留。结果表明，肉鸡比雉鸡更容易残留马杜霉素。不仅在整个停药期间，而且在停药结束后 5 天，所有鸡组织（肌肉/大腿和胸部/肫、肝、心、肾、脾、肺）都对马杜霉素呈阳性（抑制区≥4 毫米）。在雉鸡中，阳性结果仅在肫、肝、心、肾、脾和肺中检测到。停药期第 5 天未检测到阳性结果，但第 6 天心脏和脾脏再次呈阳性。
