Kennedy D G, Blanchflower W J, O'Dornan B C
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
Food Addit Contam. 1997 Jan;14(1):27-33. doi: 10.1080/02652039709374494.
Polyclonal antibodies against the coccidiostat maduramicin were produced by the immunization of rabbits with a conjugate between maduramicin and human serum albumin. The antibodies exhibited minimal cross-reactivity with structurally similar ionophoric coccidiostats (monensin, salinomycin, narasin and laslocid). An enzyme-linked immunosorbent assay (ELISA) was developed for maduramicin in poultry tissue using these antibodies. The ELISA had a limit of detection of approximately 0.02 ng/g in tissue (ng/ml in serum) and a limit of determination of 1.0 ng/g in tissue (ng/ml in serum). The half-life of maduramicin was measured in broiler chickens that had been treated with a therapeutic dose of maduramicin for 14 days. Following withdrawal of the medicated feed, the mean half-lives of maduramicin were 20, 39 and 13 h in liver, muscle and serum, respectively. At the recommended withdrawal period (5 days) the mean residual maduramicin concentration in liver was 1.6 ng/g, in muscle was 1.5 ng/g and in serum was 0.05 ng/ml.
通过用马杜霉素与人血清白蛋白的偶联物免疫兔子，制备了抗球虫药马杜霉素的多克隆抗体。这些抗体与结构相似的离子载体类抗球虫药（莫能菌素、盐霉素、那拉菌素和拉沙洛西）的交叉反应性极小。利用这些抗体开发了一种用于检测家禽组织中马杜霉素的酶联免疫吸附测定（ELISA）方法。该ELISA方法对组织（血清中为ng/ml）中马杜霉素的检测限约为0.02 ng/g，定量限为1.0 ng/g（血清中为ng/ml）。在接受治疗剂量马杜霉素处理14天的肉鸡中测定了马杜霉素的半衰期。停止投喂含药饲料后，马杜霉素在肝脏、肌肉和血清中的平均半衰期分别为20小时、39小时和13小时。在推荐的停药期（5天），肝脏中马杜霉素的平均残留浓度为1.6 ng/g，肌肉中为1.5 ng/g，血清中为0.05 ng/ml。
