Interdisciplinary Division of Science and Technology, Graduate School, Shinshu University, Ueda 386-8567, Japan.
J Biomed Mater Res A. 2011 Jun 1;97(3):272-80. doi: 10.1002/jbm.a.33054. Epub 2011 Mar 25.
In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering.
在这项研究中，通过一种有效的钙磷（Ca-P）交替浸泡方法，制备了一种新型的静电纺丝丝素/纳米羟基磷灰石（nHA）生物复合材料的组织工程支架材料。nHA 成功地在再生丝素纳米纤维上作为基底在几分钟内形成，无需任何预处理。使用场发射扫描电子显微镜（FESEM）分析了非矿化和矿化纳米纤维的形态。使用 X 射线衍射（XRD）分析了 nHA 的结晶相。傅立叶变换红外（FTIR）分光光度计和热重分析（TGA）分别用于确定丝素/nHA 生物复合材料纳米纤维中存在的官能团类型和 nHA 的量。还通过成骨样 MC3T3-E1 细胞系研究了这种新型纳米纤维生物复合材料支架的成骨活性。矿化纳米纤维上的碱性磷酸酶（ALP）活性等细胞功能得到改善。所有这些结果表明，这种丝素/nHA 生物复合材料支架材料可能是一种有前途的骨组织工程生物材料。
