Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar (MP) 470 003, India.
Curr Drug Targets. 2011 Sep;12(10):1478-97. doi: 10.2174/138945011796818135.
Dendrimers, by virtue of their therapeutic value, have recently generated enormous interest among biomedical scientists. Advancement of dendrimeric nano-architecture with well defined size, shape and controlled exterior functionality embraces promise in biomedical and pharmaceutical applications such as drug delivery, solubilization, DNA transfection and diagnosis. Highly branched, monodisperse, stable molecular level and low polydispersity with micelle-like behavior possessing nano-scale container property distinguish these structures as inimitable and optimum carrier for those applications. Dendrimers has been evaluated for delivery of different types of bioactives inside the cells. Different types of techniques are being used for exploring dendrimer safety and efficacy via cell cytotoxicity assays, cell uptake studies by fluorescent microscopy, cell line studies, flow cytometry, gamma scintigraphy and confocal microscopy; are being used over a decade. Among these, cell line studies are widely used for ex vivo characterization of dendrimers and other nanocarriers. Cell lines are homogeneous population of cells, stable after mitosis, and have an unlimited capacity for division. This review focuses on the use of different cell line studies including anticancer drugs, anti-HIV drugs, anti-inflammatory drugs, anti-tubercular-drugs, photodynamic therapy, hormonal therapy employing dendritic nanocarrier.
树状聚合物由于其治疗价值，最近在生物医学科学家中引起了极大的兴趣。具有明确尺寸、形状和受控外部功能的树状纳米结构的进步在生物医药和制药应用中具有广阔的前景，如药物输送、增溶、DNA 转染和诊断。高度支化、单分散、稳定的分子水平和低多分散性，具有胶束样行为，具有纳米级容器特性，这些结构作为这些应用的独特和最佳载体。树状聚合物已被评估用于将不同类型的生物活性物质递送到细胞内。为了通过细胞毒性测定、荧光显微镜下的细胞摄取研究、细胞系研究、流式细胞术、γ闪烁和共聚焦显微镜研究来探索树状聚合物的安全性和功效，已经使用了不同类型的技术超过十年。其中，细胞系研究广泛用于树状聚合物和其他纳米载体的体外特性研究。细胞系是细胞的同质群体，有丝分裂后稳定，并且具有无限的分裂能力。这篇综述重点介绍了使用不同的细胞系研究，包括抗癌药物、抗 HIV 药物、抗炎药物、抗结核药物、光动力疗法和激素疗法，使用树状纳米载体。
