Haque Md Emdadul, Kawaguchiand Kentaro, Komatsu Setsuko
National Institute of Crop Science, Tsukuba, 305-8518, Japan.
Protein Pept Lett. 2011 Sep;18(9):912-24. doi: 10.2174/092986611796011455.
Hypoxia caused by waterlogging results in a severe loss of crop production. At the primary stage of wheat development, the seminal roots have strategies to survive under hypoxia through alternative metabolism coupling root anatomical modification. The present study used a model system of lysigenous aerenchymatous seminal roots from a representative seedling stage of wheat to elucidate the root physiology in response to soil hypoxia. Seminal roots characteristic with lysigenous aerenchyma tissues were developed in pot cultures for 7 days under two hypoxic conditions, water depths of 15 cm below and 3 cm above the soil surface. Proteins from the roots were separated using two-dimensional polyacrylamide gel electrophoresis and identified using mass spectrometry. The results showed that approximately 345 distinct protein spots were detected by 2-DE, 29 spots changed in the expression levels between the control and two hypoxic plants, and 10 spots exhibited a reproducible up- or down regulated fluctuation. The up-regulated proteins were thought to be involved in alteration in energy and redox status, defense responses and cell wall turnover. These results suggest the effects of soil hypoxia on the activity of the identified up-regulated proteins and their roles in alternative respiration and cell degeneration in wheat in order to gain metabolic adjustment under hypoxia stress.
涝渍造成的缺氧会导致作物产量严重损失。在小麦发育的初期，胚根具有通过替代代谢与根系解剖结构改变来在缺氧条件下存活的策略。本研究使用来自小麦代表性幼苗期的溶生性通气组织胚根的模型系统，来阐明根系对土壤缺氧的生理响应。在两种缺氧条件下，即土壤表面以下15厘米和以上3厘米的水深条件下，在盆栽培养中培养7天，以发育出具有溶生性通气组织的胚根。使用二维聚丙烯酰胺凝胶电泳分离根中的蛋白质，并使用质谱进行鉴定。结果表明，通过双向电泳检测到约345个不同的蛋白质斑点，29个斑点在对照和两种缺氧植物之间的表达水平发生了变化，10个斑点表现出可重复的上调或下调波动。上调的蛋白质被认为参与了能量和氧化还原状态的改变、防御反应和细胞壁更新。这些结果表明了土壤缺氧对已鉴定的上调蛋白质活性的影响及其在小麦交替呼吸和细胞退化中的作用，以便在缺氧胁迫下进行代谢调节。
