Haque Md Emdadul, Abe Fumitaka, Mori Masahiko, Oyanagi Atsushi, Komatsu Setsuko, Kawaguchi Kentaro
NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
NARO Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
J Plant Physiol. 2014 May 1;171(8):602-9. doi: 10.1016/j.jplph.2013.12.003. Epub 2014 Mar 20.
We examined the role of pathogenesis-related protein TaBWPR-1.2 in the context of molecular and physiological responses of wheat (Triticum aestivum) seminal roots under waterlogging stress. Two cDNAs corresponding to the TaBWPR-1.2 gene, TaBWPR-1.2#2 and TaBWPR-1.2#13 were cloned from seminal roots. These cDNAs were predicted to encode proteins of 173 and 172 amino acids, respectively. In a time-course experiment, TaBWPR-1.2 gene expression was highest in whole seminal roots after 1 day of waterlogging treatment and higher than the control for at least 10 days; significantly increased protein abundance was observed after 7 days of waterlogging. Drought, another abiotic stress, did not influence TaBWPR-1.2 gene expression in wheat seminal roots at 5-d-old seedlings. Tissue-specific studies revealed that the highest TaBWPR-1.2 gene expression and protein levels were in the aerenchymatous root zone. TaBWPR-1.2 expression in seminal roots was also increased by the signalling molecules 1-aminocyclopropane-1-carboxylic acid (ACC; an ethylene precursor), H2O2, jasmonic acid (JA), and nitric oxide (NO); however, treatment with abscisic acid (ABA), salicylic acid (SA), and ethanol did not alter its expression. Interestingly, aerenchyma formation in the seminal root cortex was induced only by ACC and H2O2. Taken together, these results indicate that TaBWPR-1.2 is a waterlogging-responsive gene that might be associated with root cortex tissue alteration in wheat plants through ACC and/or H2O2 regulatory mechanisms.
我们研究了病程相关蛋白TaBWPR-1.2在渍水胁迫下小麦（Triticum aestivum）种子根分子和生理反应中的作用。从种子根中克隆了与TaBWPR-1.2基因对应的两个cDNA，即TaBWPR-1.2#2和TaBWPR-1.2#13。预计这些cDNA分别编码173和172个氨基酸的蛋白质。在一项时间进程实验中，渍水处理1天后，TaBWPR-1.2基因在整个种子根中的表达最高，并且至少10天高于对照；渍水7天后观察到蛋白质丰度显著增加。干旱，另一种非生物胁迫，对5日龄幼苗小麦种子根中的TaBWPR-1.2基因表达没有影响。组织特异性研究表明，TaBWPR-1.2基因表达和蛋白质水平最高的部位是通气组织根区。信号分子1-氨基环丙烷-1-羧酸（ACC；乙烯前体）、H2O2、茉莉酸（JA）和一氧化氮（NO）也能增加种子根中TaBWPR-1.2的表达；然而，脱落酸（ABA）、水杨酸（SA）和乙醇处理并没有改变其表达。有趣的是，仅ACC和H2O2能诱导种子根皮层通气组织的形成。综上所述，这些结果表明TaBWPR-1.2是一个对渍水响应的基因，可能通过ACC和/或H2O2调节机制与小麦植株根皮层组织改变有关。
