Department of Animal Science, University of California, Davis, One Shields Ave, Davis, CA 95616, USA.
Mol Ecol Resour. 2011 Sep;11(5):784-5. doi: 10.1111/j.1755-0998.2011.03011.x. Epub 2011 Mar 28.
We have developed species-specific TaqMan assays for two California fish species, the threatened delta smelt (Hypomesus transpacificus) and the introduced wakasagi smelt (Hypomesus nipponensis). The assays are capable of correctly identifying each species with 100% accuracy, with no cross-species amplification. We anticipate these assays will prove useful for future scientific studies requiring genetic species identification (e.g. predation of smelt) or monitoring (e.g. detection of delta smelt near water diversions).
我们开发了两种加利福尼亚鱼类物种的特异性 TaqMan 检测方法，即受到威胁的尖吻鲈（Hypomesus transpacificus）和引入的日本七鳃鳗（Hypomesus nipponensis）。这些检测方法能够以 100%的准确率正确识别每种物种，不存在交叉物种扩增。我们预计这些检测方法将在未来需要遗传物种鉴定（例如尖吻鲈的捕食）或监测（例如在水转移附近检测到尖吻鲈）的科学研究中证明是有用的。
