Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, Beijing, China.
J Gastroenterol Hepatol. 2011 Jul;26(7):1207-12. doi: 10.1111/j.1440-1746.2011.06733.x.
Serum Golgi protein 73 (sGP73) is a novel and promising biomarker for hepatocellular carcinoma (HCC). However, there are few reports on the pattern of GP73 expression in HCC and the relationship of this expression to clinicopathologic features of patients. This study aimed to investigate the expression of GP73 and it correlation with clinical parameters.
We examined GP73 expression in HCC and adjacent paracarcinomatous liver (PCL) tissue in 36 HCC patients, and took 14 normal liver (NL) samples from hepatic hemangioma patients. Western blot analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) were used for analyses.
GP73 expression in HCC was significantly higher than in the corresponding PCL and NL samples at both protein and mRNA levels (P < 0.001). The elevated level of GP73 protein was strongly associated with tumor size, vein invasion, and tumor differentiation, suggesting augmented tumor invasion and metastasis. However, there was no association between GP73 expression and patient survival.
Significant overexpression of GP73 at both protein and mRNA levels along with overexpression of GP73 protein is associated with aggressive behavior of HCC, but not overall patient survival. Further research is needed to determine the potential of GP73 as a therapeutic target.
血清高尔基糖蛋白 73（sGP73）是肝细胞癌（HCC）的一种新型有前途的生物标志物。然而，关于 HCC 中 GP73 表达模式及其与患者临床病理特征的关系的报道很少。本研究旨在探讨 GP73 的表达及其与临床参数的相关性。
我们检测了 36 例 HCC 患者的 HCC 和相邻癌旁肝（PCL）组织中的 GP73 表达，并从肝血管瘤患者中采集了 14 个正常肝（NL）样本。采用 Western blot 分析和实时定量逆转录聚合酶链反应（qRT-PCR）进行分析。
在蛋白和 mRNA 水平上，HCC 中的 GP73 表达均明显高于相应的 PCL 和 NL 样本（P<0.001）。GP73 蛋白水平升高与肿瘤大小、静脉侵犯和肿瘤分化密切相关，提示肿瘤侵袭和转移增强。然而，GP73 表达与患者生存之间没有关联。
GP73 在蛋白和 mRNA 水平上的显著过度表达以及 GP73 蛋白的过度表达与 HCC 的侵袭性行为相关，但与患者总体生存无关。需要进一步研究确定 GP73 作为治疗靶点的潜力。
