自由活动恒河猴腹腔内和皮下温度的比较。
A comparison of intraperitoneal and subcutaneous temperature in freely moving rhesus macaques.
机构信息
Committee on Neurobiology of Addictive Disorders, SP30-2400, 10550 North Torrey Pines Road, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Physiol Behav. 2011 Jul 6;103(5):440-4. doi: 10.1016/j.physbeh.2011.03.023. Epub 2011 Apr 5.
RATIONALE
The remote measurement of body temperature with radiotelemetry provides a minimally invasive and robust method for larger experimental animals such as Old World monkeys. Existing literature encompasses data using intraperitoneal (IP) and subcutaneous (SC) implantation locations which may affect inferences about body temperature.
METHODS
The body temperatures of four adult male rhesus monkeys were monitored with radiotelemetry devices implanted both IP and SC in each subject. Animals were recorded at 5 min intervals for 5 months with the two transmitters being used in sequence on a weekly basis. Additional challenge with d-methamphetamine (0.32 mg/kg; i.m.) was conducted to compare the magnitude of the hyperthermic response measured IP and SC.
RESULTS
Normal daily temperatures differed by about 0.5-0.8°C across implant locations with IP temperature consistently higher. The difference was consistent across the circadian cycle and when compared 1, 3 or 5 months after surgical implantation. The magnitude of the hyperthermia response to methamphetamine was about 0.75°C when measured with either IP or SC implants.
CONCLUSIONS
The study shows that data derived from the two major implantation locations used in existing literature are likely to be comparable.
原理
无线电遥测技术对体温的远程测量为大实验动物（如旧世界猴）提供了一种微创且可靠的方法。现有文献涵盖了使用腹腔内（IP）和皮下（SC）植入位置的数据，这可能会影响对体温的推断。
方法
使用植入每个研究对象的 IP 和 SC 的无线电遥测设备监测四只成年雄性恒河猴的体温。动物每周以 5 分钟的间隔记录 5 个月，两个发射器顺序使用。用 d-苯丙胺（0.32mg/kg；肌内注射）进行额外的挑战，以比较 IP 和 SC 测量的发热反应的幅度。
结果
植入部位的正常每日体温差异约为 0.5-0.8°C，其中 IP 温度始终较高。这种差异在昼夜节律周期内以及在手术后植入 1、3 或 5 个月后是一致的。用 IP 或 SC 植入物测量时，苯丙胺引起的发热反应幅度约为 0.75°C。
结论
该研究表明，现有文献中使用的两种主要植入位置的数据可能具有可比性。
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