Department of Microbiology, University of Szeged, Közép fasor, Szeged, Hungary.
Fungal Genet Biol. 2011 Jul;48(7):696-703. doi: 10.1016/j.fgb.2011.03.006. Epub 2011 Apr 5.
The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.
卷枝毛霉（Mucor circinelloides）积累β-胡萝卜素作为主要类胡萝卜素化合物。在这项研究中，检验了一些通用异戊二烯途径早期基因对提高该真菌类胡萝卜素产量的适用性。克隆了异戊烯焦磷酸异构酶基因（ipi），并与编码法呢基焦磷酸合酶（isoA）和香叶基香叶基焦磷酸合酶（carG）的基因一起用于过表达研究。转化实验表明，从类胡萝卜素生产的角度来看，该途径的第一个瓶颈是由 carG 基因控制的步骤，但 ipi 和 isoA 基因的过表达也有助于前体的可用性。用这些异戊二烯基因与细菌β-胡萝卜素酮化酶基因一起转化，产生了能够生产角黄素和虾青素的毛霉菌株。
