Department of Clinical Chemistry, Microbiology, and Immunology, Laboratory for Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Belgium.
Am J Obstet Gynecol. 2011 May;204(5):450.e1-7. doi: 10.1016/j.ajog.2010.12.061. Epub 2011 Mar 27.
Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase.
One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test.
Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase.
Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.
唾液酸酶和阴道加德纳菌的存在被认为是细菌性阴道病的生物标志物。唾液酸酶与不良妊娠结局有关。我们对阴道加德纳菌分离株进行了基因分型，评估了唾液酸酶编码基因的存在和多样性，并测定了唾液酸酶的产生。
通过随机扩增多态性脱氧核糖核酸（RAPD）对 134 株阴道加德纳菌分离株进行基因分型，并对扩增核糖体脱氧核糖核酸限制性分析（ARDRA）选择的 29 株分离株进行基因分型。我们开发了阴道加德纳菌唾液酸酶定量聚合酶链反应，并通过滤膜斑点试验评估唾液酸酶的产生。
RAPD 和 ARDRA 可将 3 种阴道加德纳菌基因型区分开来。只有 2 种基因型编码并产生唾液酸酶。
阴道加德纳菌分离株存在 3 种基因型，且基因型与唾液酸酶的产生之间存在明显的联系。可以假设唾液酸酶的产生与（有症状的）细菌性阴道病和生物膜的产生之间存在联系。
