Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
Fertil Steril. 2011 Jun;95(7):2435.e5-7. doi: 10.1016/j.fertnstert.2011.02.048. Epub 2011 Mar 27.
To describe a case with repeated total fertilization failure due to oocyte defect.
Case report.
University hospital.
PATIENT(S): A patient with repeated total fertilization failure.
INTERVENTION(S): Assisted oocyte activation, intracytoplasmic sperm injection (ICSI) with calcium ionophore oocyte activation, donor semen, and donor oocytes.
MAIN OUTCOME MEASURE(S): Fertilization, pregnancy, and live birth.
RESULT(S): Five cycles of IVF/ICSI were performed. In the first IVF cycle, 19 oocytes failed to fertilize. In the second ICSI attempt, 12 oocytes were retrieved and were not fertilized. In the third and fourth natural cycles, donor semen was used for IVF and ICSI, respectively, yet the oocytes could not be fertilized even by assisted activation. Then donor oocytes were used in the fifth cycle by ICSI. All five donated oocytes fertilized normally, and three embryos were transferred on day 3. Clinical pregnancy was confirmed, and a healthy girl weighing 3,200 g was delivered at 39 weeks of gestation by cesarean section.
CONCLUSION(S): In cases of repeated fertilization failure caused by oocyte defects, oocyte donation seems to be a good choice for patients who wish to become pregnant.
描述一例因卵母细胞缺陷导致反复完全受精失败的病例。
病例报告。
大学医院。
反复完全受精失败的患者。
辅助卵母细胞激活、钙离子载体卵母细胞激活的卵胞浆内单精子注射（ICSI）、供精和供卵。
受精、妊娠和活产。
进行了 5 个 IVF/ICSI 周期。在第一个 IVF 周期中，有 19 个卵母细胞未能受精。在第二次 ICSI 尝试中，取回了 12 个卵母细胞，但未受精。在第三和第四个自然周期中，分别使用供精进行 IVF 和 ICSI，但即使通过辅助激活，卵母细胞也无法受精。然后在第五个周期中通过 ICSI 使用了供体卵母细胞。所有 5 个捐赠的卵母细胞均正常受精，第 3 天移植了 3 个胚胎。临床妊娠得到确认，并通过剖宫产分娩了一名 39 周妊娠、体重 3200 克的健康女婴。
对于因卵母细胞缺陷导致反复受精失败的病例，对于希望怀孕的患者来说，卵母细胞捐赠似乎是一个不错的选择。
