Parmegiani Lodovico, Fabbri Raffaella, Cognigni Graciela Estela, Bernardi Silvia, Pocognoli Patrizia, Filicori Marco
Reproductive Medicine Unit, GynePro Medical Centers, University of Bologna, Bologna, Italy.
Fertil Steril. 2008 Nov;90(5):2014.e7-10. doi: 10.1016/j.fertnstert.2008.01.106. Epub 2008 Apr 9.
To report a live birth after the transfer of a single blastocyst derived from a human oocyte cryopreserved for 5 years.
Case report.
Private assisted reproduction center.
PATIENT(S): A 39-year-old woman with tubal infertility and her partner with male-related infertility.
INTERVENTION(S): Oocyte cryopreservation (in 1.5 mol/L 1,2-propanediol and 0.3 mol/L sucrose by slow freezing-rapid thawing protocol) when the patient was 34 years old. Oocyte thawing after 5 years of cryostorage. Insemination by intracytoplasmic sperm injection of the three best surviving oocytes, according to the Italian law regulating assisted reproductive technology.
MAIN OUTCOME MEASURE(S): Oocyte survival after thawing. Fertilization, cleavage, and embryo development into blastocyst stage.
RESULT(S): Four of six mature (metaphase II) frozen oocytes survived after thawing. Three of them were injected: these three oocytes were fertilized, and one developed into a five-cell embryo on day 3. This embryo developed into blastocyst and was transferred on day 6. A healthy female neonate weighing 3,410 g was born.
CONCLUSION(S): After 5 years of storage in liquid nitrogen, cryopreserved oocytes with a slow cooling-rapid thawing protocol can develop in vitro to blastocyst stage and produce a live birth.
报告一例由冷冻保存5年的人类卵母细胞发育而来的单个囊胚移植后活产的病例。
病例报告。
私立辅助生殖中心。
一名39岁输卵管性不孕女性及其患有男性相关不育症的伴侣。
患者34岁时进行卵母细胞冷冻保存（采用慢速冷冻 - 快速解冻方案，置于1.5 mol/L 1,2 - 丙二醇和0.3 mol/L蔗糖中）。冷冻保存5年后进行卵母细胞解冻。根据意大利辅助生殖技术相关法律，对3个存活最好的卵母细胞进行卵胞浆内单精子注射授精。
解冻后卵母细胞存活情况。受精、卵裂以及胚胎发育至囊胚阶段的情况。
6个成熟（中期II）冷冻卵母细胞解冻后有4个存活。其中3个进行了注射：这3个卵母细胞受精，1个在第3天发育为5细胞胚胎。该胚胎发育为囊胚并在第6天进行移植。一名体重3410 g的健康女婴出生。
采用慢速冷冻 - 快速解冻方案冷冻保存的卵母细胞在液氮中保存5年后可在体外发育至囊胚阶段并实现活产。
