Xiao Li, Wang Guixue, Jiang Tao, Tang Chaojun, Wu Xue, Sun Tin
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, China.
J Appl Biomater Biomech. 2011 Sep-Dec;9(3):193-8. doi: 10.5301/JABB.2011.6475.
The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.
In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.
The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.
CD133 antibody has the potential application for EPCs capture.
本研究旨在筛选可用于抗体捕获支架的内皮祖细胞（EPCs）特异性黏附基质。
在本研究中，在流动腔室的剪切应力下观察EPCs在含有三种不同抗体（VEGFR-2、CD34、CD133）的不同基质上的黏附情况。分别测量一氧化氮（NO）释放、细胞增殖和EPCs的保留率。
结果表明，一定范围内的剪切应力可促进EPCs的增殖和NO分泌。在相同剪切应力下，EPCs在含CD133抗体的基质上的黏附比在其他基质上更强。
CD133抗体在捕获EPCs方面具有潜在应用价值。
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