Mohan P F, Ihnen J S, Levin B E, Cleary M P
Hormel Institute, University of Minnesota, Austin 55912.
J Nutr. 1990 Sep;120(9):1103-14. doi: 10.1093/jn/120.9.1103.
Previous studies showed that administration of dehydroepiandrosterone (DHEA) to lean and genetically obese Zucker rats reduced body weight. In the present experiments, the effect of DHEA treatment in rats with diet-induced obesity was evaluated. In experiment 1, male Sprague-Dawley rats (300 g) were fed a nonpurified diet (reference group) or a condensed milk-corn oil nonpurified diet [diet-induced obese (DIO) rats] for 7 wk. Then, 0.6% DHEA was included in the food of one-half of the DIO rats (DIO + DHEA rats). After 6 wk, DIO rats weighed 23% more and had greater fat pad weights, cell size and cell number than reference and DIO + DHEA rats. Brown fat mitochondrial respiration was similar in all groups. DIO rats had higher serum cholesterol and triacylglycerol concentrations than reference and DIO + DHEA rats. DIO + DHEA rats had lower serum insulin levels than DIO and reference rats. In experiment 2, male Sprague-Dawley rats (460 g) were fed either the nonpurified diet or the condensed milk diet for 8 wk. Condensed milk-fed rats were then divided into DIO and diet-resistant groups. One-half of the rats in each group were fed 0.6% DHEA for 2 wk. Body weights and serum glucose, insulin, triacylglycerol and triiodothyronine levels were lowered by DHEA treatment in all groups. Liver mitochondrial state 3 respiration rates per gram and per liver and peroxisomal beta-oxidation were higher in DHEA-treated than in control rats. In DIO rats, DHEA treatment appears to interfere with hyperplastic adipose tissue growth. In this strain of rats, DHEA appears to have hypolipidemic and hypoinsulinemic effects.
先前的研究表明，给瘦的和遗传性肥胖的 Zucker 大鼠施用脱氢表雄酮（DHEA）可减轻体重。在本实验中，评估了 DHEA 治疗对饮食诱导肥胖大鼠的影响。在实验 1 中，将雄性 Sprague-Dawley 大鼠（300 克）喂食非纯化饮食（参考组）或炼乳-玉米油非纯化饮食[饮食诱导肥胖（DIO）大鼠]7 周。然后，将 0.6%的 DHEA 添加到一半的 DIO 大鼠（DIO + DHEA 大鼠）的食物中。6 周后，DIO 大鼠的体重比参考组和 DIO + DHEA 大鼠重 23%，脂肪垫重量、细胞大小和细胞数量也更大。所有组的棕色脂肪线粒体呼吸相似。DIO 大鼠的血清胆固醇和三酰甘油浓度高于参考组和 DIO + DHEA 大鼠。DIO + DHEA 大鼠的血清胰岛素水平低于 DIO 和参考大鼠。在实验 2 中，将雄性 Sprague-Dawley 大鼠（460 克）喂食非纯化饮食或炼乳饮食 8 周。然后将喂食炼乳的大鼠分为 DIO 和抗饮食组。每组一半的大鼠喂食 0.6%的 DHEA 2 周。DHEA 治疗使所有组的体重以及血清葡萄糖、胰岛素、三酰甘油和三碘甲状腺原氨酸水平降低。DHEA 处理组每克肝脏和整个肝脏的肝线粒体状态 3 呼吸率以及过氧化物酶体β-氧化均高于对照大鼠。在 DIO 大鼠中，DHEA 治疗似乎会干扰增生性脂肪组织的生长。在这种品系的大鼠中，DHEA 似乎具有降血脂和降胰岛素作用。
