Singh Ravindra P, Kang Da-Yeon, Choi Jeong-Woo
Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):408-12. doi: 10.1166/jnn.2011.3271.
Cyclic voltammetry (CV) has been used to investigate the electrochemical behavior of a glutathione (GSH) self assembled monolayer on modified gold electrodes (Bio-SAM). The GSH monolayer exhibits an influence on electrode surface activity. Electrochemically immobilized dsDNA onto a Cyt c/GSH-SAM/Au electrode, which is useful for the fabrication of a nanobiosensing device. The immobilized Cyt c followed by dsDNA immobilized films maintained its surface activity and finally dsDNA/Cyt c/GSH-SAM/Au electrode, targeted for the detection of toxicants. The films were characterized by CV, DPV, and AFM. The differential pulse voltammetry (DPV) technique was applied to detect three kinds of common toxins, 2-aminoanthracene (2-AA), 3-bromobenzanthrone (3-BBA) and bisphenol A (BPhA). The electrochemical signals showed good inverse relationship with the increase of concentrations of toxicants. Our proposed system based on electrochemical method with nanoscale film technology can be applied at highly sensitive biosensor for detecting various toxic chemicals.
循环伏安法（CV）已被用于研究修饰金电极（生物自组装单层膜，Bio-SAM）上谷胱甘肽（GSH）自组装单层的电化学行为。GSH单层对电极表面活性有影响。将双链DNA（dsDNA）电化学固定在细胞色素c/Cyt c/GSH-SAM/Au电极上，这对纳米生物传感装置的制造很有用。固定化的细胞色素c随后是固定化dsDNA的膜，保持了其表面活性，最终得到用于检测毒物的dsDNA/Cyt c/GSH-SAM/Au电极。通过循环伏安法（CV）、差分脉冲伏安法（DPV）和原子力显微镜（AFM）对这些膜进行了表征。应用差分脉冲伏安法（DPV）技术检测三种常见毒素，即2-氨基蒽（2-AA）、3-溴苯并蒽酮（3-BBA）和双酚A（BPhA）。电化学信号与毒物浓度的增加呈现出良好的反比关系。我们提出的基于电化学方法和纳米级薄膜技术的系统可应用于高灵敏度生物传感器，用于检测各种有毒化学物质。
