Faculty of Chemistry, Kharazmi University, Tehran, Iran.
Faculty of Chemistry, Kharazmi University, Tehran, Iran.
Talanta. 2015 Jan;131:460-6. doi: 10.1016/j.talanta.2014.08.009. Epub 2014 Aug 11.
The intercalative and electrostatic interaction of morphine with double-stranded DNA (ds-DNA), which was immobilized onto mercapto-benzaldehyde-modified Au electrode, was employed for designing a sensitive biosensor. The interaction of morphine with the immobilized ds-DNA onto the electrode surface has been studied by differential pulse voltammetry (DPV). Under the optimum conditions, a linear dependence for the morphine concentration in the range of 0.05-500 μmol L(-1) and its oxidation signal were observed and a detection limit of 0.01 μmol L(-1) for the morphine was obtained. The reproducibility and applicability of the analysis to real samples were also investigated and results demonstrated that this DNA biosensor could be utilized for the sensitive, rapid, simple, and cost effective determination of morphine in urine and blood plasma samples.
将吗啡与固定在巯基苯甲醛修饰的 Au 电极上的双链 DNA（ds-DNA）的插入和静电相互作用用于设计一种灵敏的生物传感器。通过差分脉冲伏安法（DPV）研究了吗啡与电极表面固定的 ds-DNA 之间的相互作用。在最佳条件下，观察到吗啡浓度在 0.05-500 μmol L(-1)范围内及其氧化信号呈线性关系，并且吗啡的检测限为 0.01 μmol L(-1)。还研究了分析对实际样品的重现性和适用性，结果表明，该 DNA 生物传感器可用于灵敏、快速、简单和经济有效地测定尿液和血浆样品中的吗啡。
