Lee Kyoung G, Kim Jin Chul, Wi Rinbok, Min Jung Sun, Ahn Jeong Keun, Kim Do Hyun
Department of Chemical and Biomolecular Engineering and Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
J Nanosci Nanotechnol. 2011 Jan;11(1):686-90. doi: 10.1166/jnn.2011.3248.
A new method is proposed for the fabrication of fluorescence-labeled and amine-modified silica nanoparticles for application as nonviral vectors in gene delivery. Highly monodisperse, stable fluorescent silica nanoparticles were prepared using 2,5-bis(5-tert-butyl-2-benzoxazolyl)thiophene and the water-in-oil microemulsion method. The green-fluorescent-protein gene can be easily combined onto the positively charged surfaces of nanoparticles to form a nanoparticle-DNA complex. The nanoparticle-DNA complex successfully passed through various barriers into the HeLa and HEK 293 K cells. The cytotoxicity of the PEI-coated and BBOT-encapsulated silica nanoparticles on both the HeLa and HEK 293T cell lines was found to be at an acceptable level for use as gene carriers when the particle concentration was below 125 microg/ml. The fluorescence intracellular images confirm the successful delivery of the nanoparticle-DNA complex and gene expression. The present work suggests the potential use of dye-incorporated silica nanoparticles in nonviral gene delivery.
本文提出了一种制备荧光标记和胺修饰二氧化硅纳米颗粒的新方法，用于作为基因递送中的非病毒载体。使用2,5-双(5-叔丁基-2-苯并恶唑基)噻吩和油包水微乳液法制备了高度单分散、稳定的荧光二氧化硅纳米颗粒。绿色荧光蛋白基因可以很容易地结合到纳米颗粒带正电的表面上，形成纳米颗粒-DNA复合物。纳米颗粒-DNA复合物成功穿过各种屏障进入HeLa和HEK 293 K细胞。当颗粒浓度低于125μg/ml时，发现聚乙烯亚胺包被和BBOT封装的二氧化硅纳米颗粒对HeLa和HEK 293T细胞系的细胞毒性处于用作基因载体的可接受水平。荧光细胞内图像证实了纳米颗粒-DNA复合物的成功递送和基因表达。目前的工作表明，染料掺入的二氧化硅纳米颗粒在非病毒基因递送中具有潜在用途。
