Plant Biotechnology Research Center, School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Biotechnol Appl Biochem. 2011 Jan-Feb;58(1):50-7. doi: 10.1002/bab.13.
Tetraploid Artemisia annua plants were successfully inducted by using colchicine, and their ploidy was confirmed by flow cytometry. Higher stomatal length but lower frequency in tetraploids were revealed and could be considered as indicators of polyploidy. The average level of artemisinin in tetraploids was increased from 39% to 56% than that of the diploids during vegetation period, as detected by high-performance liquid chromatography-evaporative light scattering detector. Gene expressions of 10 key enzymes related to artemisinin biosynthetic pathway in different ploidy level were analyzed by semiquantitative polymerase chain reaction and significant upregulation of FPS, HMGR, and artemisinin metabolite-specific Aldh1 genes were revealed in tetraploids. Slight increased expression of ADS was also detected. Our results suggest that higher artemisinin content in tetraploid A. annua may result from the upregulated expression of some key enzyme genes related to artemisinin biosynthetic pathway.
利用秋水仙素成功诱导四倍体黄花蒿植株，并通过流式细胞术对其倍性进行鉴定。结果表明，四倍体植株的气孔长度更高，但频率更低，可作为多倍体的指标。通过高效液相色谱-蒸发光散射检测器检测到，在生长期间，四倍体青蒿素的平均含量比二倍体增加了 39%至 56%。通过半定量聚合酶链反应分析不同倍性水平下与青蒿素生物合成途径相关的 10 种关键酶的基因表达，结果表明在四倍体中 FPS、HMGR 和青蒿素代谢物特异性 Aldh1 基因的表达显著上调，ADS 的表达也略有增加。研究结果表明，四倍体黄花蒿中较高的青蒿素含量可能是由于与青蒿素生物合成途径相关的一些关键酶基因的表达上调所致。
