抗人CD3和CD4单克隆抗体的质量控制
Quality control of anti human CD3 and CD4 monoclonal antibodies.
作者信息
Leerling M F, Vaessen L M, Reubsaet C H, Weimar W, Ettekoven H, Marsman F R, Kreeftenberg J G
机构信息
Laboratory for Control of Bacterial Vaccines, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
出版信息
Dev Biol Stand. 1990;71:191-200.
By testing two monoclonal antibodies (MoAb), we tried out two methods, which might be suitable for quality control of anti human T cell MoAb. RIV6 and RIV9 were investigated by flow cytometry and Enzyme-linked Immunosorbent Assay (ELISA). The characteristics, specificity and biological activity of RIV6 and RIV 9 were determined and compared respectively with other CD4 specific MoAb, OKT4, OKT4a, LEU3a and the CD3 specific MoAb OKT3 and LEU4 by flow cytometry. An ELISA was set up to quantify mouse IgG3 antibody. Specificity, plate variability and day to day effects were determined. The results illustrate that ELISA and flow cytometry are valuable tools in the quality control of these antibodies.
通过检测两种单克隆抗体（MoAb），我们尝试了两种可能适用于抗人T细胞MoAb质量控制的方法。采用流式细胞术和酶联免疫吸附测定（ELISA）对RIV6和RIV9进行研究。通过流式细胞术分别测定并比较了RIV6和RIV 9的特性、特异性及生物学活性，同时与其他CD4特异性MoAb、OKT4、OKT4a、LEU3a以及CD3特异性MoAb OKT3和LEU4进行比较。建立了ELISA法以定量小鼠IgG3抗体，并测定其特异性、板间变异性及日间效应。结果表明，ELISA和流式细胞术是这些抗体质量控制中的重要工具。
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