School of Nutrition and Health Sciences, College of Public Health and Nutrition, Taipei Medical University, Taiwan, China.
World J Gastroenterol. 2011 Feb 28;17(8):1063-70. doi: 10.3748/wjg.v17.i8.1063.
To investigate the antioxidative status of patients with alcoholic liver disease (ALD) in southeastern Taiwan.
Our study comprised 27 patients with ALD recruited from Taitung Mackay Memorial Hospital, located in southeastern Taiwan. Patients with ALD included 12 non-aborigines (12 men) and 15 aborigines (11 men and 4 women). According to the severity of ALD, patients with ALD included 10 with hepatitis (9 men and 1 woman) and 17 with cirrhosis (14 men and 3 women). Twenty-two age- and gender-matched healthy adults served as the control group in this study. Venous blood (10 mL) of each subject was drawn into EDTA-containing tubes after 8 h overnight fasting.
Compared to the control group, patients with ALD showed significantly lower erythrocytic catalase (11.1 ± 0.7 U/mg Hb vs 8.0 ± 0.7 U/mg Hb, P < 0.05) and superoxide dismutase (9.5 ± 1.6 U/mg Hb vs 3.0 ± 0.2 U/mg Hb, P < 0.05) activities. Furthermore, the erythrocytic reduced glutathione/oxidized glutathione ratio was significantly lower in ALD patients than that in the control group (38.1 ± 5.4 vs 15.7 ± 1.9, P < 0.05). The results revealed that patients with ALD experienced more oxidative stress than those in the control group. The non-aboriginal, but not the aboriginal, ALD group had higher erythrocytic glutathione peroxidase (GPX) activity than that in the control group (46.1 ± 7.8 U/g Hb vs 27.9 ± 2.2 U/g Hb, P < 0.05). Hepatitis, but not cirrhosis, ALD patients had higher erythrocytic GPX activity than that in the control group (44.3 ± 8.6 U/g Hb vs 27.9 ± 2.2 U/g Hb, P < 0.05).
Our results indicate that both ethnicity and the severity of ALD may cause different erythrocytic antioxidative enzyme activities especially GPX activity.
探讨台湾东南部地区酒精性肝病（ALD）患者的抗氧化状态。
本研究共纳入 27 例来自台湾东南部的台东马偕纪念医院的 ALD 患者。ALD 患者包括 12 名非原住民（12 名男性）和 15 名原住民（11 名男性和 4 名女性）。根据 ALD 的严重程度，ALD 患者包括 10 名肝炎（9 名男性和 1 名女性）和 17 名肝硬化（14 名男性和 3 名女性）患者。22 名年龄和性别匹配的健康成年人作为对照组。所有受试者均在禁食 8 小时后抽取静脉血（10mL）至含 EDTA 的试管中。
与对照组相比，ALD 患者的红细胞过氧化氢酶（11.1±0.7 U/mgHb 比 8.0±0.7 U/mgHb，P<0.05）和超氧化物歧化酶（9.5±1.6 U/mgHb 比 3.0±0.2 U/mgHb，P<0.05）活性显著降低。此外，ALD 患者的红细胞还原型谷胱甘肽/氧化型谷胱甘肽比值明显低于对照组（38.1±5.4 比 15.7±1.9，P<0.05）。结果表明，ALD 患者比对照组经历了更多的氧化应激。非原住民，而非原住民 ALD 组的红细胞谷胱甘肽过氧化物酶（GPX）活性高于对照组（46.1±7.8 U/gHb 比 27.9±2.2 U/gHb，P<0.05）。肝炎，而非肝硬化，ALD 患者的红细胞 GPX 活性高于对照组（44.3±8.6 U/gHb 比 27.9±2.2 U/gHb，P<0.05）。
我们的结果表明，种族和 ALD 的严重程度可能导致不同的红细胞抗氧化酶活性，特别是 GPX 活性。
