Van de Casteele M, Zaman Z, Zeegers M, Servaes R, Fevery J, Nevens F
Department of Hepatology, Catholic University of Leuven, Leuven, Belgium.
Aliment Pharmacol Ther. 2002 May;16(5):985-92. doi: 10.1046/j.1365-2036.2002.01255.x.
Enhanced production of reactive oxygen species may play a pathogenic role in alcoholic liver injury.
To investigate whether various antioxidant parameters in blood are affected in different stages of alcoholic liver disease and how specific the changes are relative to non-alcoholic cirrhosis.
Patients with alcohol abuse without cirrhosis (n=14), with alcoholic cirrhosis [Child-Pugh scores A (n=9), B (n=5) and C (n=18)] and with non-alcoholic cirrhosis [Child-Pugh score C (n=6)] and healthy controls (n=13) were studied. Levels of reduced glutathione and glutathione peroxidase activity in blood, erythrocytic superoxide dismutase activity and carotenoids, alpha-tocopherol and malondialdehyde in plasma were measured.
Levels of reduced glutathione were significantly decreased in Child-Pugh score C cirrhotics, alcoholic or not in origin, whereas oxidized glutathione and glutathione peroxidase activity were not affected. Superoxide dismutase activity and alpha-tocopherol levels were not significantly different in the various groups. Carotenoid levels were significantly lower in alcoholic cirrhotics (Child-Pugh score C) vs. controls. Malondialdehyde levels were elevated only in cirrhotics Child-Pugh score C, alcoholic or non-alcoholic.
Levels of reduced glutathione and malondialdehyde reflect the degree of liver impairment, more than the relation with alcohol intake. Decreases in several antioxidant levels are not specific to alcoholic liver injury.
活性氧物质生成增加可能在酒精性肝损伤中起致病作用。
研究酒精性肝病不同阶段血液中的各种抗氧化参数是否受到影响，以及这些变化相对于非酒精性肝硬化的特异性如何。
对无肝硬化的酒精滥用患者（n = 14）、酒精性肝硬化患者[Child-Pugh评分A（n = 9）、B（n = 5）和C（n = 18）]、非酒精性肝硬化患者[Child-Pugh评分C（n = 6）]以及健康对照者（n = 13）进行研究。检测血液中还原型谷胱甘肽水平和谷胱甘肽过氧化物酶活性，以及血浆中红细胞超氧化物歧化酶活性、类胡萝卜素、α-生育酚和丙二醛水平。
Child-Pugh评分C级的肝硬化患者，无论是否为酒精性病因，其还原型谷胱甘肽水平均显著降低，而氧化型谷胱甘肽和谷胱甘肽过氧化物酶活性未受影响。各组中超氧化物歧化酶活性和α-生育酚水平无显著差异。酒精性肝硬化患者（Child-Pugh评分C级）的类胡萝卜素水平显著低于对照组。仅Child-Pugh评分C级的肝硬化患者丙二醛水平升高，包括酒精性和非酒精性患者。
还原型谷胱甘肽和丙二醛水平反映肝脏损伤程度，甚于与酒精摄入量的关系。几种抗氧化剂水平降低并非酒精性肝损伤所特有。
