Matsuzaki T, Shimizu Y, Yokokura T
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Med Microbiol Immunol. 1990;179(3):161-8. doi: 10.1007/BF00202393.
The augmentation of the antimetastatic effect of heat-killed cells of Lactobacillus casei YIT9018 (LC9018) on Lewis lung carcinoma (3LL) in C57BL/6 mice by presensitization (priming) with LC9018 was examined. Intralesional injection of LC9018 into 3LL-bearing mice inhibited both the growth of the primary tumors and the formation of lung metastases, and this effect was significantly augmented by subcutaneous injection of LC9018 before the tumor inoculation. In the LC9018-primed mice, intraperitoneal administration of LC9018 into syngeneic hosts after priming induced a high level of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in the peritoneal cavity. At this time, T cells of the spleen cells from the LC9018-primed mice proliferated and produced IL-2 when co-cultured with LC9018 as antigen in vitro. Also, the phenotype of these T cells was found to be L3T4+ and Ly-2.2- T cells by analysis by flow cytometry. These results suggest that LC9018-reactive helper T (Th) cells were induced by the priming and subsequent challenge with LC9018, and that IL-2 or IFN-gamma, which was produced by the activated LC9018-reactive Th cells, augmented a host immune response resulting the antitumor activity.
研究了通过用干酪乳杆菌YIT9018（LC9018）预致敏（启动）来增强热灭活的LC9018对C57BL / 6小鼠Lewis肺癌（3LL）的抗转移作用。向荷3LL小鼠瘤内注射LC9018可抑制原发性肿瘤的生长和肺转移的形成，并且在肿瘤接种前皮下注射LC9018可显著增强这种作用。在经LC9018启动的小鼠中，启动后向同基因宿主腹腔内注射LC9018可在腹腔内诱导高水平的白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）。此时，经LC9018启动的小鼠脾细胞中的T细胞在体外与作为抗原的LC9018共培养时会增殖并产生IL-2。此外，通过流式细胞术分析发现这些T细胞的表型为L3T4 +和Ly-2.2- T细胞。这些结果表明，通过用LC9018启动和随后激发可诱导出对LC9018有反应的辅助性T（Th）细胞，并且由活化的对LC9018有反应的Th细胞产生的IL-2或IFN-γ增强了宿主免疫反应，从而产生抗肿瘤活性。
