Shimizu Y, Fujiwara H, Ueda S, Wakamiya N, Kato S, Hamaoka T
Eur J Immunol. 1984 Sep;14(9):839-43. doi: 10.1002/eji.1830140913.
The present study investigates the role of vaccinia virus-reactive helper T cells in causing enhanced induction of syngeneic tumor immunity. Vaccinia virus-reactive helper T cell activity capable of inducing the augmented generation of cytotoxic T lymphocyte (CTL) or antibody responses was generated in C3H/HeN mice by inoculating i.p. live virus. Immunization of these mice with vaccinia virus-infected syngeneic X5563 plasmacytoma or MH134 hapatoma cells led to augmented induction of immune resistance against the challenge with corresponding viable tumor cells when compared with the incidence of resistance observed in control mice not primed to vaccinia virus. In vitro cytotoxicity tests utilizing spleen cells and serum from mice which resulted in the augmented tumor resistance by virus help have revealed that spleen cells from C3H/HeN mice immune to the X5563 plasmacytoma exhibited appreciable anti-X5563 CTL activity, whereas serum from these mice failed to display any antibody response. In contrast, MH134-immune mice exhibited potent anti-MH134 antibody, but not CTL responses. Such an anti-tumor CTL or antibody response augmented by vaccinia virus-reactive helper T cells was found to be tumor specific. These results are discussed in the context of (a) the functional diversity of tumor antigens, and (b) mechanisms of virus help that are involved in various forms of augmented induction of syngeneic tumor immunity.
本研究调查了痘苗病毒反应性辅助性T细胞在增强同基因肿瘤免疫诱导中的作用。通过腹腔注射活病毒，在C3H/HeN小鼠中产生了能够诱导细胞毒性T淋巴细胞（CTL）增强生成或抗体反应的痘苗病毒反应性辅助性T细胞活性。与未用痘苗病毒致敏的对照小鼠中观察到的抗性发生率相比，用痘苗病毒感染的同基因X5563浆细胞瘤或MH134肝癌细胞免疫这些小鼠，可增强对相应活肿瘤细胞攻击的免疫抗性诱导。利用因病毒辅助而导致肿瘤抗性增强的小鼠的脾细胞和血清进行的体外细胞毒性试验表明，对X5563浆细胞瘤免疫的C3H/HeN小鼠的脾细胞表现出明显的抗X5563 CTL活性，而这些小鼠的血清未显示任何抗体反应。相反，对MH134免疫的小鼠表现出强效的抗MH134抗体，但没有CTL反应。发现由痘苗病毒反应性辅助性T细胞增强的这种抗肿瘤CTL或抗体反应具有肿瘤特异性。在（a）肿瘤抗原的功能多样性和（b）参与同基因肿瘤免疫各种形式增强诱导的病毒辅助机制的背景下讨论了这些结果。
