Laboratory of Protozoology, Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China.
J Eukaryot Microbiol. 2011 May-Jun;58(3):242-8. doi: 10.1111/j.1550-7408.2011.00536.x. Epub 2011 Mar 30.
The morphology, morphogenesis, and phylogeny of Diophrys parappendiculata n. sp., a large marine ciliate isolated from the coastal waters of Daya Bay, southern China, were investigated. This new species is characterized by a combination of its large size, appendiculata-pattern of ciliature, and bipartite adoral zone of membranelles. The main stages of morphogenesis during binary fission were also recorded and described. Comparisons of morphological characteristics with similar congeners support the validity of the new species. The small subunit rRNA gene sequence of D. parappendiculata is 96.3-99.94% similar to those of four other congeners; it differs in four nucleotides from that of Diophrys appendiculata (i.e. structural similarity was 99.94%). Phylogenetic analysis indicates that D. parappendiculata n. sp. falls into the Diophrys clade and is most closely related to the well-known D. appendiculata.
本研究调查了采自中国南部大亚湾近岸海域的大型海洋纤毛虫 Parappendiculata diophrys n. sp. 的形态、形态发生和系统发育。该新种的特征是体型较大，纤毛呈 appendiculata 模式，口前区膜质纤毛二分体。还记录和描述了二分体分裂过程中的主要形态发生阶段。与相似近缘种的形态特征比较支持该新种的有效性。Parappendiculata diophrys 的小亚基 rRNA 基因序列与其他四个近缘种的相似度为 96.3-99.94%；与 Diophrys appendiculata 的差异为 4 个核苷酸（即结构相似度为 99.94%）。系统发育分析表明，Parappendiculata diophrys n. sp. 属于 Diophrys 进化枝，与著名的 Diophrys appendiculata 最为密切相关。
