Department of Pharmaceutical Toxicology, Faculty of Pharmacy, University of Inonu, Malatya, Turkey.
Hum Exp Toxicol. 2011 Dec;30(12):1986-94. doi: 10.1177/0960327111404909. Epub 2011 Mar 30.
The aim of this study was to investigate the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a persistent environmental pollutant, on the percentage of T-cell subsets and B-lymphocyte and effectiveness of curcumin, β-myrcene (myrcene) and 1,8-cineole (cineol) on this toxicity in rats. Rats (n = 112) were divided randomly into 8 equal groups. One group was kept as control and given corn oil as carrier. TCDD was orally administered at the dose of 2 µg/kg/week. Curcumin, myrcene and cineol were orally administered by gavages at the doses of 100, 200 and 100 mg/kg/day, respectively, dissolved in corn oil with and without TCDD. The blood samples were taken from half of the rats on day 30 and from the rest on day 60 for the determination of lymphocyte subsets (CD3(+), CD4(+), CD8(+), CD161(+), CD45RA, CD4(+)CD25(+) and total lymphocyte). The results indicated that although TCDD significantly (p < 0.05) decreased the percentage of CD3(+), CD4(+), CD161(+), CD45RA, CD4(+)CD25(+) and total lymphocyte, it caused a significant increase in the percentage of CD8(+) cells. In contrast, curcumin, myrcene and cineol significantly decreased CD8(+) cells levels but increased CD3(+), CD4(+), CD161(+), CD45RA, CD4(+)CD25(+) and total lymphocyte cells populations. The beneficial effects of curcumin, myrcene and cineol and the toxic effects of TCDD were increased at day 60 compared to day 30. In conclusion, curcumin, myrcene and cineol showed immunomodulatory effects and eliminated TCDD-induced immune suppressive effects in rats.
本研究旨在探讨持久性环境污染物 2,3,7,8-四氯二苯并对二恶英（TCDD）对 T 细胞亚群和 B 淋巴细胞的百分比的毒性作用，以及姜黄素、β-月桂烯（月桂烯）和 1,8-桉油醇（桉油醇）对大鼠这种毒性的作用。将 112 只大鼠随机分为 8 组，一组作为对照，给予玉米油作为载体。TCDD 以 2μg/kg/周的剂量口服给药。姜黄素、月桂烯和桉油醇分别以 100、200 和 100mg/kg/天的剂量经灌胃给药，溶于玉米油中，同时给予和不给予 TCDD。第 30 天从一半大鼠的血液样本中，第 60 天从其余大鼠的血液样本中提取淋巴细胞亚群（CD3(+)、CD4(+)、CD8(+)、CD161(+)、CD45RA、CD4(+)CD25(+)和总淋巴细胞）进行测定。结果表明，虽然 TCDD 显著（p < 0.05）降低了 CD3(+)、CD4(+)、CD161(+)、CD45RA、CD4(+)CD25(+)和总淋巴细胞的百分比，但却显著增加了 CD8(+)细胞的百分比。相反，姜黄素、月桂烯和桉油醇显著降低 CD8(+)细胞水平，但增加 CD3(+)、CD4(+)、CD161(+)、CD45RA、CD4(+)CD25(+)和总淋巴细胞群体。与第 30 天相比，第 60 天姜黄素、月桂烯和桉油醇的有益作用以及 TCDD 的毒性作用增加。总之，姜黄素、月桂烯和桉油醇具有免疫调节作用，可消除 TCDD 诱导的大鼠免疫抑制作用。
